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Journal Article

Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons56884

Reusch,  Thorsten B. H.
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons56875

Rauch,  Gisep
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons56756

Kalbe,  Martin
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;
Research Group Parasitology, Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Reusch, T. B. H., Rauch, G., & Kalbe, M. (2004). Polymorphic microsatellite loci for the trematode Diplostomum pseudospathaceum. Molecular Ecology Notes, 4(4), 577-579. doi:10.1111/j.1471-8286.2004.00740.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-DA4A-A
Abstract
We present primers for five polymorphic microsatellite loci in the eye fluke Diplostomum pseudospathaceum (Trematoda), a widely distributed parasite with a complex life cycle used as a model for parasitology and fish immunology. The loci were identified using a GA/CT-enriched genomic library by subtractive hybridization with magnetic particles. All five loci were highly polymorphic, displaying 17 to 61 alleles and heterozygosities ranging from 0.53 to 0.92. We isolated populations of parasites within the first (snail) and second (fish) intermediate host and found small but significant genetic differentiation (FST= 0.012) between the two life stages of the parasite.