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Measurement of exoenzymatic activity in streambed sediments using methylumbelliferyl-substrates

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons56815

Marxsen,  Jürgen
Limnological River Station Schlitz, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons57008

Witzel,  Karl-Paul
Department Microbial Ecology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Marxsen, J., & Witzel, K.-P. (1990). Measurement of exoenzymatic activity in streambed sediments using methylumbelliferyl-substrates. Archiv für Hydrobiologie/Ergebniss der Limnologie, 34, 21-28.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-CA27-A
Abstract
4-Methylumbelliferyl-substrates were used to measure the activity of extracellular β-D-glucosidase and phosphatase in small central European streams. The substrates could be used for stream water, sandy sediments, and coarser artificial substrata. Although β-D-glucosidase activity per unit surface area was about ten-times higher in coarse particulate sediments than in sandy sediments, maximum activity per unit volume was observed in sandy sediments. This means that for the stream as a whole, the greater proportion of the exoenzymatic cleavage activty occurs in the sandy streambed deposits.