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Measurement of Low-level Extracellular Enzyme Activity in Natural Waters Using Fluorigenic Model Substrates

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons56724

Hendel,  Björn
Limnological River Station Schlitz, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons56815

Marxsen,  Jürgen
Limnological River Station Schlitz, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Hendel, B., & Marxsen, J. (1997). Measurement of Low-level Extracellular Enzyme Activity in Natural Waters Using Fluorigenic Model Substrates. Acta Hydrochimica et Hydrobiologica, 25(5), 253-258.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-C8ED-B
Abstract
Activities of the extracellular enzymes β-D-glucosidase, phosphatase, and leucine aminopeptidase were investigated in unpolluted waters to test the suitability of fluorigenic model substrates for measuring enzyme activities in water samples expected to sustain low levels of activity. The waters tested were surface running waters, shallow groundwaters, and deep groundwaters from water extraction wells. Enzyme activity was detected in all the waters tested, although some of the hydrolytic rates were at the lower limit of current analytical capability. Rates varied between 1.1 and 291 nmol/(L⋅h) for β-D-glucosidase, between 9.2 and 320 nmol/(L⋅h) for phosphatase, and between 5.8 and 1560 nmol/(L⋅h) for leucine aminopeptidase. The method thus proved to be suitable for measuring low levels of extracellular enzyme activity in natural waters, but in any case, it is necessary to adapt the technique to the type of water investigated.