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Biotin-Streptavidin Sensor Surface: a Versatile Platform for Performing DNA Hybridization Interactions

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Kambhampati,  D.
MPI for Polymer Research, Max Planck Society;

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Knoll,  Wolfgang
MPI for Polymer Research, Max Planck Society;

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Citation

Kambhampati, D., & Knoll, W. (2002). Biotin-Streptavidin Sensor Surface: a Versatile Platform for Performing DNA Hybridization Interactions. In D. Möbius, & R. Miller (Eds.), Organized Monolayers and Assemblies: Structure, Processes and Function (pp. 279-316). Elsevier.


Cite as: https://hdl.handle.net/11858/00-001M-0000-000F-6725-D
Abstract
The properties of the biotin-streptavidin sensor system such as surface topology, regeneration of probes and non-specific interaction effects are investigated in detail by using DNA-DNA hybridization interactions as the test system. Surface Plasmon Resonance (SPR) and SPR enhanced fluorescence spectroscopy (SPRFS) detection schemes were widely employed to monitor the effect of probe/target length, mismatches and temperature on the hybridization kinetics. 1:1 Langmuir kinetic models were found to be sufficient to describe the hybridization interactions.