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Fusion of small unilamellar vesicles onto laterally mixed self- assembled monolayers of thiolipopeptides

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons47625

Baumgart,  T.
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48224

Kreiter,  Maximilian
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48260

Lauer,  H.
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48487

Naumann,  Renate
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48528

Offenhäusser,  Andreas
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48198

Knoll,  Wolfgang
MPI for Polymer Research, Max Planck Society;

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Zitation

Baumgart, T., Kreiter, M., Lauer, H., Naumann, R., Jung, G., Jonczyk, A., et al. (2003). Fusion of small unilamellar vesicles onto laterally mixed self- assembled monolayers of thiolipopeptides. Journal of Colloid and Interface Science, 258(2), 298-309.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-000F-628C-F
Zusammenfassung
Monolayers of the thiolipopeptide NH2-Cys-Ala-Ser-Ala-Ala-Ser- Ser-Ala-Pro-Ser-Ser-(Myr)Lys(Myr)-OH (III) were formed on gold surfaces by self-assembly, mixed with a lateral spacer of the same peptide composition, NH2-Cys-Ala-Ser-Ala-Ala-Ser-Ser- AlaPro-Ser-Ser-Lys-OH (I). Different mixing ratios were employed ranging from 0.1 to 1, corresponding to 10-100% thiolipopeptide. These self-assembled monolayers (SAMs) were then exposed to a suspension of liposomes with the aim of forming lipid bilayers as a function of the mixing ratio. A clear optimum with respect to homogeneity and electrical properties of the membranes was obtained in the middle region (0.5) of mixing ratio, as revealed by surface plasmon resonance spectroscopy, impedance spectroscopy, and fluorescence microscopy. The combination of these methods was shown to be a powerful tool, although a true lipid bilayer was not obtained. Instead, vesicle adsorption was shown to be the predominant process, and FRAP (fluorescence recovery after photobleaching) measurements showed that the films were not fluid on the micrometer length scale. (C) 2003 Elsevier Science (USA). All rights reserved.