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Journal Article

Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons48302

Liebermann,  T.
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48198

Knoll,  Wolfgang
MPI for Polymer Research, Max Planck Society;

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Citation

Liebermann, T., & Knoll, W. (2003). Parallel multispot detection of target hybridization to surface-bound probe oligonucleotides of different base mismatch by surface-plasmon field-enhanced fluorescence microscopy. Langmuir, 19(5), 1567-1572.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-626B-A
Abstract
We introduce surface-plasmon field-enhanced fluorescence microscopy for the on-line determination of hybridization reactions between targets from solution to complementary probe oligonucleotides which are surface-bound to a sensor substrate in a matrix format. This way, the parallel and real-time detection of the association and dissociation kinetics (rate constants) of hybrids of different mismatch situations can be quantitatively analyzed.