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Zeitschriftenartikel

No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons47957

Graf,  Robert
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48014

Hehn,  M.
MPI for Polymer Research, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons48786

Spiess,  Hans Wolfgang
MPI for Polymer Research, Max Planck Society;

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Zitation

Schiffer, I. B., Schreiber, W. G., Graf, R., Schreiber, E. M., Jung, D., Rose, D. M., et al. (2003). No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI. Bioelectromagnetics, 24(4), 241-250.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-000F-6216-6
Zusammenfassung
The purpose of this study was to examine whether exposure to magnetic fields (MFs) relevant for magnetic resonance imaging (MRI) in clinical routine influences cell cycle progression in two tumor cell lines in vitro. HL60 and EA2 cells were exposed to four types of MFs: (i) static MF of 1.5 and 7.05 T, (ii) extremely low frequency magnetic gradient fields (ELFMGFs) with ± 10 mT/m and 100 Hz, as well as ± 100 mT/m and 100 Hz, (iii) pulsed high frequency MF in the radiofrequency (RF) range (63.6 MHz, 5.8 μT), and (iv) a combination of (i-iii). Exposure periods ranged from 1 to 24 h. Cell cycle distribution (G0/G1, S, and G2/M phases) was analyzed by flow cytometry. Cell cycle analysis did not reveal differences between the exposed and the control cells. As expected, positive controls with irradiated (8 Gy) HL60 and EA2 cells showed a strong G2/M arrest. Using conditions that are relevant for patients during MRI, no influence of MFs on cell cycle progression was observed in these cell lines. Care was taken to control secondary parameters of influence, such as vibration by the MR scanner or temperature to avoid false positive results.