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  Examining Synaptotagmin 1 Function in Dense Core Vesicle Exocytosis under Direct Control of Ca2+

Soerensen, J. B., Fernández-Chacón, R., Südhof, T. C., & Neher, E. (2003). Examining Synaptotagmin 1 Function in Dense Core Vesicle Exocytosis under Direct Control of Ca2+. Journal of General Physiology, 122(3), 265-276. doi:10.1085/jgp.200308855.

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Other : Examining Synaptotagmin 1 Function in Dense Core Vesicle Exocytosis under Direct Control of Ca2+

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Soerensen, J. B.1, Author           
Fernández-Chacón , R., Author
Südhof, T. C., Author
Neher, E.2, Author           
Affiliations:
1Research Group of Molecular Mechanisms of the Exocytosis, MPI for biophysical chemistry, Max Planck Society, ou_578584              
2Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the KD of Ca2+-dependent phospholipid binding to the double C2A-C2B domain of synaptotagmin. Using photolysis of caged calcium and capacitance measurements we found that secretion from mutant cells had lower secretory rates, longer secretory delays, and a higher intracellular Ca2+-threshold for secretion due to a twofold increase in the apparent KD of the Ca2+ sensor for fast exocytosis. Single amperometric fusion events were unchanged. We conclude that Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis.

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Language(s): eng - English
 Dates: 2003-09
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1085/jgp.200308855
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Title: Journal of General Physiology
  Other : J. Gen. Physiol.
  Abbreviation : JGP
Source Genre: Journal
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Publ. Info: Rockefeller University Press
Pages: - Volume / Issue: 122 (3) Sequence Number: - Start / End Page: 265 - 276 Identifier: ISSN: 0022-1295
CoNE: https://pure.mpg.de/cone/journals/resource/954925413895