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  The repulsive guidance molecule RGMa is involved in the formation of afferent connections in the dentate gyrus.

Brinks, H., Conrad, S., Vogt, J., Oldekamp, J., Sierra, A., Deitinghoff, L., et al. (2004). The repulsive guidance molecule RGMa is involved in the formation of afferent connections in the dentate gyrus. The Journal of Neuroscience, 24(15), 3862-3869. Retrieved from http://www.jneurosci.org/cgi/reprint/24/15/3862.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0012-EDBE-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-E296-8
Genre: Journal Article

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 Creators:
Brinks, H., Author
Conrad, S., Author
Vogt, J., Author
Oldekamp, J., Author
Sierra, A., Author
Deitinghoff, L., Author
Bechmann, I., Author
Alvarez-Bolado, G.1, Author
Heimrich, B., Author
Monnier, P. P., Author
Mueller, B. K., Author
Skutella, T., Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: axon; dentate; development; guidance; hippocampus; neuron
 Abstract: In the developing dentate gyrus, afferent fiber projections terminate in distinct laminas. This relies on an accurately regulated spatiotemporal network of guidance molecules. Here, we have analyzed the functional role of the glycosylphosphatidylinositol (GPI)-anchored repulsive guidance molecule RGMa. In situ hybridization in embryonic and postnatal brain showed expression of RGMa in the cornu ammonis and hilus of the hippocampus. In the dentate gyrus, RGM immunostaining was confined to the inner molecular layer, whereas the outer molecular layers targeted by entorhinal fibers remained free. To test the repulsive capacity of RGMa, different setups were used: the stripe and explant outgrowth assays with recombinant RGMa, and entorhino-hippocampal cocultures incubated either with a neutralizing RGMa antibody (Ab) or with the GPI anchor-digesting drug phosphatidylinositol-specific phospholipase C. Entorhinal axons were clearly repelled by RGMa in the stripe and outgrowth assays. After disrupting the RGMa function, the specific laminar termination pattern in entorhino-hippocampal cocultures was lost, and entorhinal axons entered inappropriate hippocampal areas. Our data indicate an important role of RGMa for the layer-specific termination of the perforant pathway as a repulsive signal that compels entorhinal fibers to stay in their correct target zone.

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Language(s): eng - English
 Dates: 2004-04-14
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 426864
URI: http://www.jneurosci.org/cgi/reprint/24/15/3862
 Degree: -

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Title: The Journal of Neuroscience
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 24 (15) Sequence Number: - Start / End Page: 3862 - 3869 Identifier: -