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  Charge controlled microfluidic formation of lipid-based single- and multicompartment systems

Haller, B., Göpfrich, K., Schröter, M., Janiesch, J.-W., Platzman, I., & Spatz, J. P. (2018). Charge controlled microfluidic formation of lipid-based single- and multicompartment systems. Lab on a Chip, 1-20. doi:10.1039/C8LC00582F.

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https://pubs.rsc.org/en/content/articlepdf/2018/lc/c8lc00582f (beliebiger Volltext)
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 Urheber:
Haller, Barbara1, 2, Autor           
Göpfrich, Kerstin1, 2, Autor           
Schröter, Martin1, 2, Autor           
Janiesch, Jan-Willi1, 2, Autor           
Platzman, Ilia1, 2, Autor           
Spatz, Joachim P.1, 2, Autor           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Schlagwörter: synthetic cell, droplet-based microfluidics, water-in-oil-droplets, giant unilamellar vesicles, fluorosurfactants, compartments
 Zusammenfassung: ABSTRACT: In this manuscript, we introduce a simple, off-the-shelf approach for the on-demand creation of giant unilamellar vesicles (GUVs) or multicompartment synthetic cell model systems in a high-throughput manner. To achieve this, we use microfluidics to encapsulate small unilamellar vesicles in block-copolymer surfactant-stabilized water-in-oil droplets. By tuning the charge of the inner droplet interface, adsorption of lipids can either be inhibited, leading to multicompartment systems, or induced, leading to the formation of a droplet-stabilized GUV. To control the charge density, we formed droplets using different molar ratios of an uncharged PEG-based fluorosurfactant and a negatively-charged PFPE carboxylic acid fluorosurfactant (Krytox). We systematically studied the transition from a multicompartment system to 3D-supported lipid bilayers as a function of lipid charge and Krytox concentration using confocal fluorescence microscopy and interfacial tension measurements. Moreover, we showed and characterized the release of assembled GUVs from the surfactant shell and the oil phase into a physiological buffer, an approach for the formation of GUVs with improved fea-tures that is high in yield compared to standard GUV formation methods. This widely applica-ble microfluidic-based technology will increase the scope of usage for GUVs as adaptable cell-like compartments in bottom-up synthetic biology applications and beyond.

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Sprache(n): eng - English
 Datum: 2018-06-072018-07-252018-07-26
 Publikationsstatus: Erschienen
 Seiten: 20
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1039/C8LC00582F
 Art des Abschluß: -

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Titel: Lab on a Chip
  Andere : Lab Chip
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: Cambridge : Royal Society of Chemistry
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 1 - 20 Identifikator: Anderer: 1473-0197
CoNE: https://pure.mpg.de/cone/journals/resource/14730197