English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Non-Canonical Hedgehog Signaling Is a Positive Regulator of the WNT Pathway and Is Required for the Survival of Colon Cancer Stem Cells

Regan, J. L., Schumacher, D., Staudte, S., Steffen, A., Haybaeck, J., Keilholz, U., et al. (2017). Non-Canonical Hedgehog Signaling Is a Positive Regulator of the WNT Pathway and Is Required for the Survival of Colon Cancer Stem Cells. Cell Reports, 21(10), 2813-2828. doi:10.1016/j.celrep.2017.11.025.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0000-F693-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-F694-3
Genre: Journal Article

Files

show Files
hide Files
:
Regan.pdf (Publisher version), 21MB
View   Save
File Permalink:
https://hdl.handle.net/21.11116/0000-0000-F695-2
Name:
Regan.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
© 2017 The Author(s)
License:
-

Locators

show
hide
Locator:
https://www.ncbi.nlm.nih.gov/pubmed/29212028 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Regan, J. L., Author
Schumacher, D., Author
Staudte, S., Author
Steffen, A., Author
Haybaeck, J., Author
Keilholz, U., Author
Schweiger, C., Author
Golob-Schwarzl, N., Author
Mumberg, D., Author
Henderson, D., Author
Lehrach, H.1, Author           
Regenbrecht, C. R. A., Author
Schafer, R., Author
Lange, M., Author
Affiliations:
1Emeritus Group of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2385697              

Content

show
hide
Free keywords: Hhat Ptch1 Shh WNT pathway cancer organoid cancer stem cell colon cancer non-canonical Hedgehog signaling
 Abstract: Colon cancer is a heterogeneous tumor driven by a subpopulation of cancer stem cells (CSCs). To study CSCs in colon cancer, we used limiting dilution spheroid and serial xenotransplantation assays to functionally define the frequency of CSCs in a panel of patient-derived cancer organoids. These studies demonstrated cancer organoids to be enriched for CSCs, which varied in frequency between tumors. Whole-transcriptome analysis identified WNT and Hedgehog signaling components to be enhanced in CSC-enriched tumors and in aldehyde dehydrogenase (ALDH)-positive CSCs. Canonical GLI-dependent Hedgehog signaling is a negative regulator of WNT signaling in normal intestine and intestinal tumors. Here, we show that Hedgehog signaling in colon CSCs is autocrine SHH-dependent, non-canonical PTCH1 dependent, and GLI independent. In addition, using small-molecule inhibitors and RNAi against SHH-palmitoylating Hedgehog acyltransferase (HHAT), we demonstrate that non-canonical Hedgehog signaling is a positive regulator of WNT signaling and required for colon CSC survival.

Details

show
hide
Language(s): eng - English
 Dates: 2017-11-062017-12-05
 Publication Status: Published online
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.celrep.2017.11.025
ISSN: 2211-1247 (Electronic)
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Maryland Heights, MO : Cell Press
Pages: - Volume / Issue: 21 (10) Sequence Number: - Start / End Page: 2813 - 2828 Identifier: ISSN: 2211-1247
CoNE: https://pure.mpg.de/cone/journals/resource/2211-1247