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  Cloning and expression of the metE gene in Escherichia coli

Chu, J., Shoeman, R. L., Hart, J., Coleman, T., Mazaitis, A., Kelker, N., et al. (1985). Cloning and expression of the metE gene in Escherichia coli. Archives of Biochemistry and Biophysics, 239(2), 467-474. doi:10.1016/0003-9861(85)90713-1.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-CF1F-6 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-CF20-3
Genre: Journal Article

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ArchBiochemBiophys_239_1985_467.pdf (Any fulltext), 2MB
 
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 Creators:
Chu, Jennifer, Author
Shoeman, Robert L.1, 2, 3, Author           
Hart, Jean, Author
Coleman, Timothy, Author
Mazaitis, Anthony, Author
Kelker, Norman, Author
Brot, Nathan, Author
Weissbach, Herbert, Author
Affiliations:
1Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              

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 Abstract: A lambda-transducing phage was isolated that contains the metE gene. This gene codes for N5-methyl-H4-folate:homocysteine methyltransferase (EC 2.1.1.14), an enzyme that catalyzes the terminal reaction in methionine biosynthesis. A 9.1-kb EcoR1 fragment of this phage, containing the metE gene, was then cloned into pBR325. This plasmid, pJ19, was used to transform Escherichia coli strain 2276, a metE mutant, and restore the MetE+ phenotype. Although the transformed cells produced large amounts of the metE protein in vivo, in vitro studies using pJ19 as template showed low synthesis of the metE protein.

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Language(s): eng - English
 Dates: 1984-10-312004-11-021985-06
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0003-9861(85)90713-1
URI: https://www.ncbi.nlm.nih.gov/pubmed/2988449
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Title: Archives of Biochemistry and Biophysics
Source Genre: Journal
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Publ. Info: New York : Academic Press
Pages: - Volume / Issue: 239 (2) Sequence Number: - Start / End Page: 467 - 474 Identifier: ISSN: 0003-9861
CoNE: https://pure.mpg.de/cone/journals/resource/991042745826956