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  Ultrastructural analysis of cytoplasmic intermediate filaments and the nuclear lamina in the mouse plasmacytoma cell line MPC-11 after the induction of vimentin synthesis

Wang, X., Willingale−Theune, J., Shoeman, R. L., Giese, G., & Traub, P. (1989). Ultrastructural analysis of cytoplasmic intermediate filaments and the nuclear lamina in the mouse plasmacytoma cell line MPC-11 after the induction of vimentin synthesis. European Journal of Cell Biology: EJCB, 50(2), 462-474. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2627942.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-84FB-0 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-84FC-F
Genre: Journal Article

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EurJCellBiol_50_1989_462.pdf (Any fulltext), 10MB
 
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 Creators:
Wang, Xiao, Author
Willingale−Theune, Julia, Author
Shoeman, Robert L.1, 2, 3, Author           
Giese, Günter4, 5, Author           
Traub, Peter, Author
Affiliations:
1Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
2Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
3Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              
4Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
5Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society, ou_1497720              

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 Abstract: We examined cytoplasmic intermediate filaments (IFs) and the nuclear lamina in cells of the mouse plasmacytoma cell line MPC-11 (lacking both IF proteins and lamins A and C) after induction of vimentin synthesis with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) by means of whole-mount immunogold electron microscopy (IEM). The technique of IEM was modified to allow analysis of the cytoskeleton and nuclear lamina of cells grown in suspension culture employing antibodies against vimentin and lamin B. IEM showed that newly synthesized vimentin assembled into IFs which formed anastomosing networks throughout the cytoplasm, radiating primarily from the nucleus. The filaments decorated by gold-conjugated antibodies appeared to make contact with the lipid-depleted nuclear envelope residue either by directly terminating on it or through an indirect link via short fibers of varying diameter. Some filaments terminated on the subunits of the nuclear pore complexes but they did not pass through the pores. In the absence of lamins A and C, lamin B formed a nuclear lamina consisting of a globular-filamentous network anchoring the nuclear pore complexes.

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Language(s): eng - English
 Dates: 1989-07-131989-09-261989-12
 Publication Status: Issued
 Pages: -
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 Rev. Type: Peer
 Identifiers: URI: http://www.ncbi.nlm.nih.gov/pubmed/2627942
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Title: European Journal of Cell Biology : EJCB
  Other : Eur. J. Cell Biol.
Source Genre: Journal
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Publ. Info: Stuttgart : Wissenschaftliche Verlagsgesellschaft.
Pages: - Volume / Issue: 50 (2) Sequence Number: - Start / End Page: 462 - 474 Identifier: ISSN: 0070-2463
CoNE: https://pure.mpg.de/cone/journals/resource/954925486755