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  Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

Laviv, T., Kim, B. B., Chu, J., Lam, A. J., Lin, M. Z., & Yasuda, R. (2016). Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins. Nature Methods, advance online publication, 989-992. doi:10.1038/nmeth.4046.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2AE0-A Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2AE1-8
Genre: Journal Article
Alternative Title : Nature Methods

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 Creators:
Laviv, Tal1, Author
Kim, Benjamin B.2, Author
Chu, Jun2, Author
Lam, Amy J.2, Author
Lin, Michael Z.2, Author
Yasuda, Ryohei1, Author
Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288              
2External Organizations, ou_persistent22              

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Free keywords: Cell signalling, Fluorescent proteins, Molecular neuroscience
 Abstract: We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.

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 Dates: 2016
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4046.html
URI: http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.4046.html
DOI: 10.1038/nmeth.4046
 Degree: -

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Title: Nature Methods
  Alternative Title : Nat Meth
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: advance online publication Sequence Number: - Start / End Page: 989 - 992 Identifier: ISBN: 1548-7091