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  Measurement of backbone hydrogen-deuterium exchange in the type III secretion system needle protein PrgI by solid-state NMR.

Chevelkov, V., Giller, K., Becker, S., & Lange, A. (2017). Measurement of backbone hydrogen-deuterium exchange in the type III secretion system needle protein PrgI by solid-state NMR. Journal of Magnetic Resonace, 283, 110-116. doi:10.1016/j.jmr.2017.08.012.

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 Creators:
Chevelkov, V., Author
Giller, K.1, Author           
Becker, S.1, Author           
Lange, A., Author
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1Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Magic-angle spinning solid-state NMR; Relax-EXSY; H/D exchange; Deuteration; Amide protons; Proton detection; PrgI; Relaxation
 Abstract: In this report we present site-specific measurements of amide hydrogen-deuterium exchange rates in a protein in the solid state phase by MAS NMR. Employing perdeuteration, proton detection and a high external magnetic field we could adopt the highly efficient Relax-EXSY protocol previously developed for liquid state NMR. According to this method, we measured the contribution of hydrogen exchange on apparent N-15 longitudinal relaxation rates in samples with differing D2O buffer content. Differences in the apparent T-1 times allowed us to derive exchange rates for multiple residues in the type III secretion system needle protein.

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Language(s): eng - English
 Dates: 2017-09-012017-10
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.jmr.2017.08.012
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Title: Journal of Magnetic Resonace
Source Genre: Journal
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Pages: - Volume / Issue: 283 Sequence Number: - Start / End Page: 110 - 116 Identifier: -