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  Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA

Kugadas, A., Wright, Q., Geddes-McAlister, J., & Gadjeva, M. (2017). Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA. Investigative Ophthalmology and Visual Science, 58(11), 4593-4600. doi:10.1167/iovs.17-22119.

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i1552-5783-58-11-4593.pdf (Publisher version), 974KB
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iovs-58-10-18_s01.pdf (Supplementary material), 51KB
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 Creators:
Kugadas, Abirami1, Author
Wright, Quentin1, Author
Geddes-McAlister, Jennifer2, Author           
Gadjeva, Mihaela1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: B-CELLS; DIVERSITY; QUANTIFICATION; COMMENSALS; IMMUNITY; DISEASE; TIMEOphthalmology; microbiota; SIgA; ocular surface proteome; 16S metagenomics;
 Abstract: PURPOSE. The purpose of this study was to evaluate mechanisms controlling secretory IgA (SIgA) production, thereby ensuring maintenance of ocular surface health. METHODS. To determine whether the presence of specific gut commensal species regulates SIgA levels and IgA transcripts in the eye-associated lymphoid tissues (EALT), specificpathogen- free (SPF) Swiss Webster (SW) mice were treated with antibiotic cocktails, germfree (GF) SW mice were reconstituted with diverse commensal gut microbiota, or monocolonized with gut-specific commensals. Proteomic profiling and quantitative real-time polymerase chain reaction (qRT-PCR) were used to quantify SIgA and IgA levels. 16S rDNA sequencing was carried out to characterize commensal microbiota. RESULTS. Commensal presence regulated ocular surface SIgA levels and mRNA IgA transcripts in EALT. Oral antibiotic cocktail intake significantly reduced gut commensal presence, while maintaining ocular surface commensal levels reduced SIgA and IgA transcripts in EALT. Analysis of gut microbial communities revealed that SPF SW mice carried abundant Bacteroides organisms when compared to SPF C57BL6/N mice, with B. acidifaciens being the most prominent species in SPF SW mice. Monocolonization of GF SW mice with B. acidifaciens, a strict gut anaerobe, resulted in significant increase of IgA transcripts in the EALT, implying generation of B-cell memory. CONCLUSIONS. These data illustrated a "gut-eye'' axis of immune regulation. Exposure of the host to gut commensal species may serve as a priming signal to generate B-cell repertoires at sites different from the gut, such as EALT, thereby ensuring broad protection.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000410944300019
DOI: 10.1167/iovs.17-22119
 Degree: -

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Title: Investigative Ophthalmology and Visual Science
Source Genre: Journal
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Publ. Info: Hagerstown, MD, etc. : Association for Research in Vision and Ophthalmology, etc.
Pages: - Volume / Issue: 58 (11) Sequence Number: - Start / End Page: 4593 - 4600 Identifier: ISSN: 0146-0404
CoNE: https://pure.mpg.de/cone/journals/resource/110978984074949