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  Low-dose cryo electron ptychography via non-convex Bayesian optimization

Pelz, P., Qiu, W. X., Bücker, R., Kassier, G., & Miller, R. J. D. (2017). Low-dose cryo electron ptychography via non-convex Bayesian optimization. Scientific Reports, 7: 9883. doi:10.1038/s41598-017-07488-y.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F364-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F365-1
Genre: Journal Article

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scientific reports_pelz.pdf (Publisher version), 6MB
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2017
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© Pelz, P. et al.
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http://dx.doi.org/10.1038/s41598-017-07488-y (Publisher version)
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 Creators:
Pelz, Philipp1, 2, 3, Author              
Qiu, Wen Xuan4, Author
Bücker, Robert2, Author
Kassier, Günther2, Author
Miller, R. J. Dwayne2, 4, Author
Affiliations:
1International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, escidoc:2266714              
2Max Planck Institute for the Structure and Dynamics of Matter, Center for Free Electron Laser Science, Luruper Chaussee 149, 22761, Hamburg, Germany, escidoc:persistent22              
3Department of Physics, University of Hamburg, Hamburg, 22761, Germany, escidoc:persistent22              
4Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, M5S 1H6, Canada, escidoc:persistent22              

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Free keywords: Cryoelectron microscopy, Imaging techniques, Phase-contrast microscopy, Scanning electron microscopy, Single-molecule biophysics
 Abstract: Electron ptychography has seen a recent surge of interest for phase sensitive imaging at atomic or near-atomic resolution. However, applications are so far mainly limited to radiation-hard samples, because the required doses are too high for imaging biological samples at high resolution. We propose the use of non-convex Bayesian optimization to overcome this problem, and show via numerical simulations that the dose required for successful reconstruction can be reduced by two orders of magnitude compared to previous experiments. As an important application we suggest to use this method for imaging single biological macromolecules at cryogenic temperatures and demonstrate 2D single-particle reconstructions from simulated data with a resolution up to 5.4 Å at a dose of 20e-2. When averaging over only 30 low-dose datasets, a 2D resolution around 3.5 Å is possible for macromolecular complexes even below 100 kDa. With its independence from the microscope transfer function, direct recovery of phase contrast, and better scaling of signal-to-noise ratio, low-dose cryo electron ptychography may become a promising alternative to Zernike phase-contrast microscopy.

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Language(s): eng - English
 Dates: 2017-02-172017-06-272017-08-292017-08-29
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-017-07488-y
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 7 Sequence Number: 9883 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/2045-2322