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Free keywords:
HUMAN SKELETAL-MUSCLE; AGE-RELATED-CHANGES; IN-VIVO; MITOCHONDRIAL
SPECIALIZATION; METABOLIC PATHWAYS; PROTEIN-SYNTHESIS; GENE-EXPRESSION;
KREBS CYCLE; RNA-SEQ; EXERCISECell Biology;
Abstract:
Skeletal muscle is a key tissue in human aging, which affects different muscle fiber types unequally. We developed a highly sensitive single muscle fiber proteomics workflow to study human aging and show that the senescence of slow and fast muscle fibers is characterized by diverging metabolic and protein quality control adaptations. Whereas mitochondrial content declines with aging in both fiber types, glycolysis and glycogen metabolism are upregulated in slow but downregulated in fast muscle fibers. Aging mitochondria decrease expression of the redox enzyme monoamine oxidase A. Slowfibers up-regulate a subset of actin and myosin chaperones, whereas an opposite change happens in fast fibers. These changes in metabolism and sarcomere quality control may be related to the ability of slow, but not fast, muscle fibers to maintain their mass during aging. We conclude that single muscle fiber analysis by proteomics can elucidate pathophysiology in a sub-type-specific manner.
