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  A Novel Cervical spinal Cord Window Preparation Allows for two-Photon Imaging of t-Cell Interactions with the Cervical spinal Cord Microvasculature during experimental Autoimmune encephalomyelitis

Jahromi, N. H., Tardent, H., Enzmann, G., Deutsch, U., Kawakami, N., Bittner, S., et al. (2017). A Novel Cervical spinal Cord Window Preparation Allows for two-Photon Imaging of t-Cell Interactions with the Cervical spinal Cord Microvasculature during experimental Autoimmune encephalomyelitis. Frontiers in immunology, 8: UNSP 406. doi:10.3389/fimmu.2017.00406.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A5FE-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A5FF-B
Genre: Journal Article

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fimmu-08-00406.pdf (Publisher version), 4MB
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© 2017 Haghayegh Jahromi, Tardent, Enzmann, Deutsch, Kawakami, Bittner, Vestweber, Zipp, Stein and Engelhardt. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
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 Creators:
Jahromi, Neda Haghayegh1, Author
Tardent, Heidi1, Author
Enzmann, Gaby1, Author
Deutsch, Urban1, Author
Kawakami, Naoto2, Author              
Bittner, Stefan1, Author
Vestweber, Dietmar1, Author
Zipp, Frauke1, Author
Stein, Jens V.1, Author
Engelhardt, Britta1, Author
Affiliations:
1external, escidoc:persistent22              
2Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, escidoc:1113547              

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Free keywords: BLOOD-BRAIN-BARRIER; SELECTIN GLYCOPROTEIN LIGAND-1; CENTRAL-NERVOUS-SYSTEM; MULTIPLE-SCLEROSIS; P-SELECTIN; IN-VIVO; CNS AUTOIMMUNITY; LESIONS; ACTIVATION; CD8(+)Immunology; cervical spinal cord window; two-photon intravital microscopy; experimental autoimmune encephalomyelitis; blood-brain barrier; T-cell migration;
 Abstract: T-cell migration across the blood-brain barrier (BBB) is a crucial step in the pathogenesis of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple scle rosis (MS). Two-photon intravital microscopy (2P-IVM) has been established as a powerful tool to study cell-cell interactions in inflammatory EAE lesions in living animals. In EAE, central nervous system inflammation is strongly pronounced in the spinal cord, an organ in which 2P-IVM imaging is technically very challenging and has been limited to the lumbar spinal cord. Here, we describe a novel spinal cord window preparation allowing to use 2P-IVM to image immune cell interactions with the cervical spinal cord microvascular endothelium during EAE. We describe differences in the angioarchitecture of the cervical spinal cord versus the lumbar spinal cord, which will entail different hemodynamic parameters in these different vascular beds. Using T cells as an example, we demonstrate the suitability of this novel methodology in imaging the post-arrest multistep T-cell extravasation across the cervical spinal cord microvessels. The novel methodology includes an outlook to the analysis of the cellular pathway of T-cell diapedesis across the BBB by establishing visualization of endothelial junctions in this vascular bed.

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Language(s): eng - English
 Dates: 2017-04-11
 Publication Status: Published online
 Pages: 18
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000398730700001
DOI: 10.3389/fimmu.2017.00406
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Title: Frontiers in immunology
  Abbreviation : Front immunol
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 8 Sequence Number: UNSP 406 Start / End Page: - Identifier: Other: 1664-3224
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/1664-3224