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  Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels

Hadden, W. J., Young, J. L., Holle, A. W., McFetridge, M., Kim, D. Y., Wijesinghe, P., et al. (2017). Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels. Proceedings of the National Academy of Sciences of the United States of America, 114(22), 5647-5652. doi:10.1073/pnas.1618239114.

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 Urheber:
Hadden, William J., Autor
Young, Jennifer L.1, 2, Autor           
Holle, Andrew W.1, 2, Autor           
McFetridge, Meg, Autor
Kim, Du Yong, Autor
Wijesinghe, Philig, Autor
Taylor-Weiner, Hermes, Autor
Wen, Jessica H., Autor
Lee, Andrew R., Autor
Bieback, Karen, Autor
Vo, Ba-Ngu, Autor
Sampson, David , Autor
Kennedy, Brendan F., Autor
Spatz, Joachim P.1, 2, Autor           
Engler, Adam J., Autor
Choi, Yu Suk, Autor
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_ persistent22              

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Schlagwörter: mechanobiology / stem cell migration / stem cell differentiation / extracellular matrix / stiffness
 Zusammenfassung: The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed a method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient at the cell–matrix interface. This simple, low-cost, robust method was used to produce polyacrylamide hydrogels with stiffness gradients of 0.5, 1.7, 2.9, 4.5, 6.8, and 8.2 kPa/mm, spanning the in vivo physiological and pathological mechanical landscape. Importantly, three of these gradients were found to be nondurotactic for human adipose-derived stem cells (hASCs), allowing the presentation of a continuous range of stiffnesses in a single well without the confounding effect of differential cell migration. Using these nondurotactic gradient gels, stiffness-dependent hASC morphology, migration, and differentiation were studied. Finally, the mechanosensitive proteins YAP, Lamin A/C, Lamin B, MRTF-A, and MRTF-B were analyzed on these gradients, providing higher-resolution data on stiffness-dependent expression and localization.

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Sprache(n): eng - English
 Datum: 2016-11-042017-04-242017-05-30
 Publikationsstatus: Erschienen
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1073/pnas.1618239114
PMC: 28507138
 Art des Abschluß: -

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Titel: Proceedings of the National Academy of Sciences of the United States of America
  Andere : Proc. Acad. Sci. USA
  Andere : Proc. Acad. Sci. U.S.A.
  Kurztitel : PNAS
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, D.C. : National Academy of Sciences
Seiten: - Band / Heft: 114 (22) Artikelnummer: - Start- / Endseite: 5647 - 5652 Identifikator: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230