English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Architecture of a transcribing-translating expressome.

Kohler, R., Mooney, R. A., Mills, D. J., Landick, R., & Cramer, P. (2017). Architecture of a transcribing-translating expressome. Science, 356(6334), 194-197. doi:10.1126/science.aal3059.

Item is

Files

show Files
hide Files
:
2424189.pdf (Publisher version), 879KB
 
File Permalink:
-
Name:
2424189.pdf
Description:
-
OA-Status:
Visibility:
Restricted (UNKNOWN id 303; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2424189_Suppl.DC1 (Supplementary material), 53KB
Name:
2424189_Suppl.DC1
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/xhtml+xml / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Kohler, R.1, Author           
Mooney, R. A., Author
Mills, D. J., Author
Landick, R., Author
Cramer, P.1, Author           
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: -
 Abstract: DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of Escherichia coli can form a defined transcribing and translating “expressome” complex. The cryo–electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.

Details

show
hide
Language(s): eng - English
 Dates: 2017-04-142017-04-14
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.aal3059
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 356 (6334) Sequence Number: - Start / End Page: 194 - 197 Identifier: -