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  Optochemokine tandem for light-control of intracellular Ca2+

Feldbauer, K., Schlegel, J., Weissbecker, J., Sauer, F., Wood, P. G., Bamberg, E., et al. (2016). Optochemokine tandem for light-control of intracellular Ca2+. PLoS One, 10(11): e0165344. doi:doi:10.1371/journal.pone.0165344.

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Feldbauer, Katrin1, Autor           
Schlegel, Jan2, Autor
Weissbecker, Juliane1, Autor           
Sauer, Frank2, Autor
Wood, Philip G.1, Autor           
Bamberg, Ernst1, 3, Autor           
Terpitz, Ulrich2, Autor
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Department of Biotechnology and Biophysics, Biocenter, Julius Maximilian University, Würzburg, Germany, ou_persistent22              
3Chemical and Pharmaceutical Sciences Department, Johann Wolfgang Goethe University, ou_persistent22              

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 Zusammenfassung: An optochemokine tandem was developed to control the release of calcium from endosomes into the cytosol by light and to analyze the internalization kinetics of G-protein coupled receptors (GPCRs) by electrophysiology. A previously constructed rhodopsin tandem was re-engineered to combine the light-gated Ca2+-permeable cation channel Channelrhodopsin-2(L132C), CatCh, with the chemokine receptor CXCR4 in a functional tandem protein tCXCR4/CatCh. The GPCR was used as a shuttle protein to displace CatCh from the plasma membrane into intracellular areas. As shown by patch-clamp measurements and confocal laser scanning microscopy, heterologously expressed tCXCR4/CatCh was internalized via the endocytic SDF1/CXCR4 signaling pathway. The kinetics of internalization could be followed electrophysiologically via the amplitude of the CatCh signal. The light-induced release of Ca2+ by tandem endosomes into the cytosol via CatCh was visualized using the Ca2+-sensitive dyes rhod2 and rhod2-AM showing an increase of intracellular Ca2+ in response to light.

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Sprache(n): eng - English
 Datum: 2016-07-292016-10-102016-10-21
 Publikationsstatus: Online veröffentlicht
 Seiten: 20
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: doi:10.1371/journal.pone.0165344
 Art des Abschluß: -

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Titel: PLoS One
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: San Francisco, CA : Public Library of Science
Seiten: - Band / Heft: 10 (11) Artikelnummer: e0165344 Start- / Endseite: - Identifikator: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850