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2H quadrupolar splittings; 2D experiments; Chiral liquid crystals; INEPT transfers; Proton relay; u/l Stereoisomers
Abstract:
We describe several homo- and heteronuclear 2D NMR strategies dedicated to the analysis of anisotropic 2H spectra of a mixture of dideuterated unlike/like stereoisomers with two remote stereogenic centers, using weakly orienting chiral liquid crystals. To this end, we propose various 2D correlation experiments, denoted “D(H)nD” or “D(H)nC” (with n = 1, 2), that involve two heteronuclear polarization transfers of INEPT-type with one or two proton relays. The analytical expressions of correlation signals for four pulse sequences reported here were calculated using the product-operators formalism for spin I = 1 and S = 1/2. The features and advantages of each scheme are presented and discussed. The efficiency of these 2D sequences is illustrated using various deuterated model molecules, dissolved in organic solutions of polypeptides made of poly-γ-benzyl-l-glutamate (PBLG) or poly-ε-carbobenzyloxy-l-lysine (PCBLL) and NMR numerical simulations.
