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  Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae

Khoo, S. K., Loll, B., Chan, W. T., Shoeman, R. L., Ngoo, L., Yeo, C. C., et al. (2007). Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae. Journal of Biological Chemistry, 282(27), 19606-19618. doi:10.1074/jbc.M701703200.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-A8AD-E Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-A8AE-C
Genre: Journal Article
Alternative Title : Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae

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 Creators:
Khoo, Seok Kooi, Author
Loll, Bernhard1, Author           
Chan, Wai Ting, Author
Shoeman, Robert L.1, Author           
Ngoo, Lena, Author
Yeo, Chew Chieng, Author
Meinhart, Anton1, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: The chromosomal pezT gene of the Gram-positive pathogen Streptococcus pneumoniae encodes a protein that is homologous to the zeta toxin of the Streptococcus pyogenes plasmid pSM19035-encoded epsilon-zeta toxin-antitoxin system. Overexpression of pezT in Escherichia coli led to severe growth inhibition from which the bacteria recovered approximately 3 h after induction of expression. The toxicity of PezT was counteracted by PezA, which is encoded immediately upstream of pezT and shares weak sequence similarities in the C-terminal region with the epsilon antitoxin. The pezAT genes form a bicistronic operon that is co-transcribed from a sigma(70)-like promoter upstream of pezA and is negatively autoregulated with PezA functioning as a transcriptional repressor and PezT as a co-repressor. Both PezA and the non-toxic PezA(2)PezT(2) protein complex bind to a palindrome sequence that overlaps the promoter. This differs from the epsilon-zeta system in which epsilon functions solely as the antitoxin and transcriptional regulation is carried out by another protein designated omega. Results from site-directed mutagenesis experiments demonstrated that the toxicity of PezT is dependent on a highly conserved phosphoryltransferase active site and an ATP/GTP nucleotide binding site. In the PezA(2)PezT(2) complex, PezA neutralizes the toxicity of PezT by blocking the nucleotide binding site through steric hindrance.

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Language(s): eng - English
 Dates: 2007-04-232007-02-272007-05-082007-05-082007-07-06
 Publication Status: Issued
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665289
DOI: 10.1074/jbc.M701703200
URI: http://www.ncbi.nlm.nih.gov/pubmed/17488720
URI: http://www.jbc.org/cgi/content/full/282/27/19606?maxtoshow%3D%26HITS%3D10%26hits%3D10%26RESULTFORMAT%3D%26author1%3Dmeinhart%26andorexactfulltext%3Dand%26searchid%3D1%26FIRSTINDEX%3D0%26sortspec%3Drelevance%26resourcetype%3DHWCIT
URI: http://www.jbc.org/cgi/reprint/282/27/19606?maxtoshow%3D%26HITS%3D10%26hits%3D10%26RESULTFORMAT%3D%26author1%3Dmeinhart%26andorexactfulltext%3Dand%26searchid%3D1%26FIRSTINDEX%3D0%26sortspec%3Drelevance%26resourcetype%3DHWCIT
Other: 6788
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Title: Journal of Biological Chemistry
  Other : J. Biol. Chem.
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 282 (27) Sequence Number: - Start / End Page: 19606 - 19618 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826