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  Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy

Besztejan, S., Keskin, S., Manz, S., Kassier, G., Bücker, R., Venegas-Rojas, D., et al. (2017). Visualization of Cellular Components in a Mammalian Cell with Liquid-Cell Transmission Electron Microscopy. Microscopy and Microanalysis. doi:10.1017/S1431927616012708.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7E14-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7E15-7
Genre: Journal Article

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https://doi.org/10.1017/S1431927616012708 (Publisher version)
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 Creators:
Besztejan, Stephanie1, 2, 3, Author              
Keskin, Sercan1, 4, Author              
Manz, Stephanie4, Author
Kassier, Günther4, Author
Bücker, Robert4, Author
Venegas-Rojas, Deybith5, Author
Trieu, Hoc K.5, Author
Rentmeister, Andrea6, Author
Miller, R. J. Dwayne3, 4, 7, Author
Affiliations:
1International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, escidoc:2266714              
2Chemistry Department, Institute for Biochemistry and Molecular Biology, University of Hamburg, Martin-Luther-King Platz 6, 20146 Hamburg, Germany, escidoc:persistent22              
3The Hamburg Centre for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, escidoc:persistent22              
4Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, Geb. 99 (CFEL), 22761 Hamburg, Germany, escidoc:persistent22              
5Institute of Microsystems Technology, Hamburg University of Technology (TUHH), Eißendorfer Straße 42, 21073 Hamburg, Germany, escidoc:persistent22              
6Institute of Biochemistry, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Strasse 2, 48149 Muenster, Germany, escidoc:persistent22              
7Departments of Chemistry and Physics, University of Toronto, 80 St George St, Toronto, ON, CanadaM5S3H6, escidoc:persistent22              

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Free keywords: Liquid-cell TEM, mammalian cells, nanofluidic cell, in situ imaging, gold nanoparticles
 Abstract: We present liquid-cell transmission electron microscopy (liquid-cell TEM) imaging of fixed and non-fixed prostate cancer cells (PC3 and LNCaP) with high resolution in a custom developed silicon nitride liquid cell. Fixed PC3 cells were imaged for 90–120 min without any discernable damage. High contrast on the cellular structures was obtained even at low electron doses (~2.5 e-/nm2 per image). The images show distinct structures of cell compartments (nuclei and nucleoli) and cell boundaries without any further sample embedding, dehydration, or staining. Furthermore, we observed dynamics of vesicles trafficking from the cell membrane in consecutive still frames in a non-fixed cell. Our findings show that liquid-cell TEM, operated at low electron dose, is an excellent tool to investigate dynamic events in non-fixed cells with enough spatial resolution (few nm) and natural amplitude contrast to follow key intracellular processes.

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Language(s): eng - English
 Dates: 2016-08-122016-12-142017-01-31
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1017/S1431927616012708
 Degree: -

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Title: Microscopy and Microanalysis
  Abbreviation : Microsc. Microanal.
Source Genre: Journal
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Publ. Info: New York, NY : Cambridge University Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1431-9276
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/991042731793414