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  Deletion of Aquaporin-4 curtails extracellular glutamate elevation in cortical spreading depression in awake mice

Enger, R., Dukefoss, D. B., Tang, W., Pettersen, K. H., Bjørnstad, D. M., Helm, P. J., et al. (2017). Deletion of Aquaporin-4 curtails extracellular glutamate elevation in cortical spreading depression in awake mice. Cerebral Cortex, 27(1), 24-33. doi:10.1093/cercor/bhw359.

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 Creators:
Enger, Rune, Author
Dukefoss, Didrik B., Author
Tang, Wannan, Author
Pettersen, Klas H., Author
Bjørnstad, Daniel M., Author
Helm, P. Johannes, Author
Jensen, Vidar, Author
Sprengel, Rolf1, Author           
Vervaeke, Koen, Author
Ottersen, Ole P., Author
Nagelhus, Erlend A., Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: AQP4, astrocyte, calcium, glia, homeostasis, IP3R2, migraine
 Abstract: Cortical spreading depression (CSD) is a phenomenon that challenges the homeostatic mechanisms on which normal brain function so critically depends. Analyzing the sequence of events in CSD holds the potential of providing new insight in the physiological processes underlying normal brain function as well as the pathophysiology of neurological conditions characterized by ionic dyshomeostasis. Here, we have studied the sequential progression of CSD in awake wild-type mice and in mice lacking aquaporin-4 (AQP4) or inositol 1,4,5-triphosphate type 2 receptor (IP3R2). By the use of a novel combination of genetically encoded sensors that a novel combination - an unprecedented temporal and spatial resolution, we show that CSD leads to brisk Ca2+ signals in astrocytes and that the duration of these Ca2+ signals is shortened in the absence of AQP4 but not in the absence of IP3R2. The decrease of the astrocytic, AQP4-dependent Ca2+ signals, coincides in time and space with a decrease in the duration of extracellular glutamate overflow but not with the initial peak of the glutamate release suggesting that in CSD, extracellular glutamate accumulation is extended through AQP4-dependent glutamate release from astrocytes. The present data point to a salient glial contribution to CSD and identify AQP4 as a new target for therapy.

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Language(s): eng - English
 Dates: 2016-10-252016-06-222016-10-292016-11-232017-01-01
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Cerebral Cortex
Source Genre: Journal
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Publ. Info: New York, NY : Oxford University Press
Pages: - Volume / Issue: 27 (1) Sequence Number: - Start / End Page: 24 - 33 Identifier: ISSN: 1047-3211
CoNE: https://pure.mpg.de/cone/journals/resource/954925592440