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  SNARE-mediated membrane fusion trajectories derived from force-clamp experiments.

Oelkers, M., Witt, H., Halder, P., Jahn, R., & Janshoff, A. (2016). SNARE-mediated membrane fusion trajectories derived from force-clamp experiments. Proceedings of the National Academy of Sciences of the United States of America, 113(46), 13051-13056. doi:10.1073/pnas.1615885113.

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 Urheber:
Oelkers, M., Autor
Witt, H., Autor
Halder, P.1, Autor           
Jahn, R.1, Autor           
Janshoff, A., Autor
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

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Schlagwörter: membrane fusion; SNARE; force spectroscopy; AFM; hydration barrier
 Zusammenfassung: Fusion of lipid bilayers is usually prevented by large energy barriers arising from removal of the hydration shell, formation of highly curved structures, and, eventually, fusion pore widening. Here, we measured the force-dependent lifetime of fusion intermediates using membrane-coated silica spheres attached to cantilevers of an atomic-force microscope. Analysis of time traces obtained from force-clamp experiments allowed us to unequivocally assign steps in deflection of the cantilever to membrane states during the SNARE-mediated fusion with solid-supported lipid bilayers. Force-dependent lifetime distributions of the various intermediate fusion states allowed us to propose the likelihood of different fusion pathways and to assess the main free energy barrier, which was found to be related to passing of the hydration barrier and splaying of lipids to eventually enter either the fully fused state or a long-lived hemifusion intermediate. The results were compared with SNARE mutants that arrest adjacent bilayers in the docked state and membranes in the absence of SNAREs but presence of PEG or calcium. Only with the WT SNARE construct was appreciable merging of both bilayers observed.

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Sprache(n): eng - English
 Datum: 2016-11-15
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1073/pnas.1615885113
 Art des Abschluß: -

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Titel: Proceedings of the National Academy of Sciences of the United States of America
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 113 (46) Artikelnummer: - Start- / Endseite: 13051 - 13056 Identifikator: -