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  The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain

Vasiljeva, L., Kim, M., Mutschler, H., Buratowski, S., & Meinhart, A. (2008). The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nature Structural and Molecular Biology, 15(8), 795-804. doi:10.1038/nsmb.1468.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002C-0A40-E Version Permalink: https://hdl.handle.net/21.11116/0000-0000-DE56-6
Genre: Journal Article

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NatStructMolBiol_15_2008_795.pdf (Any fulltext), 849KB
 
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http://www.nature.com/nsmb/journal/v15/n8/pdf/nsmb.1468.pdf (Any fulltext)
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 Creators:
Vasiljeva, Lidia, Author
Kim, Minkyu, Author
Mutschler, Hannes1, Author           
Buratowski, Stephen, Author
Meinhart, Anton1, Author           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: RNA polymerase II (Pol II) in Saccharomyces cerevisiae can terminate transcription via several pathways. To study how a mechanism is chosen, we analyzed recruitment of Nrd1, which cooperates with Nab3 and Sen1 to terminate small nucleolar RNAs and other short RNAs. Budding yeast contains three C-terminal domain (CTD) interaction domain (CID) proteins, which bind the CTD of the Pol II largest subunit. Rtt103 and Pcf11 act in mRNA termination, and both preferentially interact with CTD phosphorylated at Ser2. The crystal structure of the Nrd1 CID shows a fold similar to that of Pcf11, but Nrd1 preferentially binds to CTD phosphorylated at Ser5, the form found proximal to promoters. This indicates why Nrd1 cross-links near 5' ends of genes and why the Nrd1-Nab3-Sen1 termination pathway acts specifically at short Pol II-transcribed genes. Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3.

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Language(s): eng - English
 Dates: 2007-12-182008-06-242008-07-272008-08-01
 Publication Status: Issued
 Pages: 10
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb.1468
URI: http://www.ncbi.nlm.nih.gov/pubmed/18660819
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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 15 (8) Sequence Number: - Start / End Page: 795 - 804 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763