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  Ataxin-10 interacts with O-linked beta-N-acetylglucosamine transferase in the brain

März, P., Stetefeld, J., Bendfeldt, K., Nitsch, C., Reinstein, J., Shoeman, R. L., et al. (2006). Ataxin-10 interacts with O-linked beta-N-acetylglucosamine transferase in the brain. The Journal of Biological Chemistry, 281(29), 20263-20270. doi:10.1074/jbc.M601563200.

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Genre: Journal Article
Alternative Title : Ataxin-10 interacts with O-linked beta-N-acetylglucosamine transferase in the brain

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JBiolChem_281_2006_20263.pdf (Any fulltext), 518KB
 
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 Creators:
März, Pia, Author
Stetefeld, Jörg, Author
Bendfeldt, Kerstin, Author
Nitsch, Cordula, Author
Reinstein, Jochen1, Author           
Shoeman, Robert L.1, Author           
Dimitriades−Schmutz, Beatrice, Author
Schwager, Martine, Author
Leiser, Dominic, Author
Özcan, Sabire, Author
Otten, Uwe, Author
Özbek, Suat, Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Modification by O-GlcNAc involves a growing number of eucaryotic nuclear and cytosolic proteins. Glycosylation of intracellular proteins is a dynamic process that in several cases competes with and acts as a reciprocal modification system to phosphorylation. O-Linked beta-N-acetylglucosamine transferase (OGT) levels are highest in the brain, and neurodegenerative disorders such as Alzheimer disease have been shown to involve abnormally phosphorylated key proteins, probably as a result of hypoglycosylation. Here, we show that the neurodegenerative disease protein ataxin-10 (Atx-10) is associated with cytoplasmic OGT p110 in the brain. In PC12 cells and pancreas, this association is competed by the shorter OGT p78 splice form, which is down-regulated in brain. Overexpression of Atx-10 in PC12 cells resulted in the reconstitution of the Atx-10-OGT p110 complex and enhanced intracellular glycosylation activity. Moreover, in an in vitro enzyme assay using PC12 cell extracts, Atx-10 increased OGT activity 2-fold. These data indicate that Atx-10 might be essential for the maintenance of a critical intracellular glycosylation level and homeostasis in the brain.

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Language(s): eng - English
 Dates: 2006-02-172006-05-182006-05-182006-07-21
 Publication Status: Issued
 Pages: 8
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 Rev. Type: Peer
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 281 (29) Sequence Number: - Start / End Page: 20263 - 20270 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1