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  Post-synthetic Spin-Labeling of RNA through Click Chemistry for PELDOR Measurements

Kerzhner, M., Abdullin, D., Wiecek, J., Matsuoka, H., Hagelueken, G., Schiermann, O., et al. (2016). Post-synthetic Spin-Labeling of RNA through Click Chemistry for PELDOR Measurements. Chemistry – A European Journal, 22, 12113-12121. doi:10.1002/chem.201601897.

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 Creators:
Kerzhner, Mark, Author
Abdullin, Dinar, Author
Wiecek, Jennifer, Author
Matsuoka, Hideto, Author
Hagelueken, Gregor, Author
Schiermann, Olav, Author
Famulok, Michael1, Author           
Affiliations:
1Max Planck Fellow Chemical Biology, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2173681              

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Free keywords: conformation analysis, EPR spectroscopy, oligonucleotides, RNA structures, spin label
 Abstract: Site‐directed spin labeling of RNA based on click chemistry is used in combination with pulsed electron‐electron double resonance (PELDOR) to benchmark a nitroxide spin label, called here dŲ . We compare this approach with another established method that employs the rigid spin label Çm for RNA labeling. By using CD spectroscopy, thermal denaturation measurements, CW‐EPR as well as PELDOR we analyzed and compared the influence of dŲ and Çm on a self‐complementary RNA duplex. Our results demonstrate that the conformational diversity of dŲ is significantly reduced near the freezing temperature of a phosphate buffer, resulting in strongly orientation‐selective PELDOR time traces of the dŲ ‐labeled RNA duplex.

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 Dates: 2016-06-14
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/chem.201601897
 Degree: -

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Project name : Chemistry at Spin Centres - Concepts, Mechanisms, Functions
Grant ID : SFB813
Funding program : Collaborative Research Centres (60803019)
Funding organization : Deutsche Forschungsgemeinschaft - DFG

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Title: Chemistry – A European Journal
  Abbreviation : Chem Eur J
Source Genre: Journal
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Publ. Info: Weinheim, Germany : VCH Verlagsgesellschaft
Pages: - Volume / Issue: 22 Sequence Number: - Start / End Page: 12113 - 12121 Identifier: ISSN: 0947-6539
CoNE: https://pure.mpg.de/cone/journals/resource/954926979058