Femtosecond structural dynamics drives the trans/cis isomerization in 
photoactive yellow protein

Pande, Kanupriya; Hutchison, Christopher D. M.; Groenhof, Gerrit; Aquila, Andy; Robinson, Josef S.; Tenboer, Jason; Basu, Shibom; Boutet, Sébastien; DePonte, Daniel P.; Liang, Mengning; White, Thomas A.; Zatsepin, Nadia A.; Yefanov, Oleksandr; Morozov, Dmitry; Oberthuer, Dominik; Gati, Cornelius; Subramanian, Ganesh; James, Daniel; Zhao, Yun; Koralek, Jake; Brayshaw, Jennifer; Kupitz, Christopher; Conrad, Chelsie; Roy-Chowdhury, Shatabdi; Coe, Jesse D.; Metz, Markus; Paulraj, Lourdu Xavier; Grant, Thomas D.; Koglin, Jason E.; Ketawala, Gihan; Fromme, Raimund; Šrajer, Vukica; Henning, Robert; Spence, John C. H.; Ourmazd, Abbas; Schwander, Peter; Weierstall, Uwe; Frank, Matthias; Fromme, Petra; Barty, Anton; Chapman, Henry N.; Moffat, Keith; van Thor, Jasper J.; Schmidt, Marius

doi:10.1126/science.aad5081

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Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein

Pande, K., Hutchison, C. D. M., Groenhof, G., Aquila, A., Robinson, J. S., Tenboer, J., et al. (2016). Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein. Science, 352(6286), 725-729. doi:10.1126/science.aad5081.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-D6E4-C Version Permalink: https://hdl.handle.net/21.11116/0000-0007-0EC1-0

Genre: Journal Article

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http://dx.doi.org/10.1126/science.aad5081 (Publisher version) Open Access status unknown

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Pande, Kanupriya^{1, 2}, Author
Hutchison, Christopher D. M.³, Author
Groenhof, Gerrit⁴, Author
Aquila, Andy⁵, Author
Robinson, Josef S.⁵, Author
Tenboer, Jason¹, Author
Basu, Shibom⁶, Author
Boutet, Sébastien⁵, Author
DePonte, Daniel P.⁵, Author
Liang, Mengning⁵, Author
White, Thomas A.², Author
Zatsepin, Nadia A.⁷, Author
Yefanov, Oleksandr², Author
Morozov, Dmitry⁴, Author
Oberthuer, Dominik², Author
Gati, Cornelius², Author
Subramanian, Ganesh⁷, Author
James, Daniel⁷, Author
Zhao, Yun⁷, Author
Koralek, Jake⁵, Author
Brayshaw, Jennifer¹, AuthorKupitz, Christopher¹, AuthorConrad, Chelsie⁶, AuthorRoy-Chowdhury, Shatabdi⁶, AuthorCoe, Jesse D.⁶, AuthorMetz, Markus², AuthorPaulraj, Lourdu Xavier^{2, 8}, Author Grant, Thomas D.⁹, AuthorKoglin, Jason E.⁵, AuthorKetawala, Gihan⁶, AuthorFromme, Raimund⁶, AuthorŠrajer, Vukica¹⁰, AuthorHenning, Robert¹⁰, AuthorSpence, John C. H.⁷, AuthorOurmazd, Abbas¹, AuthorSchwander, Peter¹, AuthorWeierstall, Uwe⁷, AuthorFrank, Matthias¹¹, AuthorFromme, Petra⁶, AuthorBarty, Anton², AuthorChapman, Henry N.^{2, 12}, AuthorMoffat, Keith^{10, 13}, Authorvan Thor, Jasper J.³, AuthorSchmidt, Marius¹, Author more..

Affiliations:
1Department of Physics, University of Wisconsin–Milwaukee, Milwaukee, WI 53211, USA, ou_persistent22
2Center for Free Electron Laser Science, Deutsches Elektronen Synchrotron, Notkestrasse 85, 22607 Hamburg, Germany, ou_persistent22
3Faculty of Natural Sciences, Department of Life Sciences, Imperial College, London SW7 2AZ, UK, ou_persistent22
4Nanoscience Center and Department of Chemistry, University of Jyväskylä, Post Office Box 35, 40014 Jyväskylä, Finland, ou_persistent22
5Linac Coherent Light Source, SLAC National Accelerator Laboratory, Sand Hill Road, Menlo Park, CA 94025, USA, ou_persistent22
6School of Molecular Sciences and Biodesign Center for Applied Structural Discovery, Arizona State University, Tempe, AZ 85287, USA, ou_persistent22
7Department of Physics, Arizona State University, Tempe, AZ 85287, USA, ou_persistent22
8International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2266714
9Hauptman-Woodward Institute, State University of New York at Buffalo, 700 Ellicott Street, Buffalo, NY 14203, USA, ou_persistent22
10Center for Advanced Radiation Sources, University of Chicago, Chicago, IL 60637, USA, ou_persistent22
11Lawrence Livermore National Laboratory, Livermore, CA 94550, USA, ou_persistent22
12Center for Ultrafast Imaging, University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22
13Department of Biochemistry and Molecular Biology and Institute for Biophysical Dynamics, University of Chicago, Chicago, IL 60637, USA, ou_persistent22

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Abstract: A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction.

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Language(s): eng - English

Dates: Submitted: 2015-09-27Accepted: 2016-04-05Published Online: 2016-05-06Date issued: 2016-05-06

Publication Status: Issued

Pages: 5

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Rev. Type: Peer

Identifiers: DOI: 10.1126/science.aad5081

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Title: Science

Source Genre: Journal

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Publ. Info: Washington, D.C. : American Association for the Advancement of Science

Pages: - Volume / Issue: 352 (6286) Sequence Number: - Start / End Page: 725 - 729 Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1