de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases.

Kuban-Jankowska, A., Gorska, M., Jaremko, L., Jaremko, M., Tuszynski, J. A., & Wozniak, M. (2015). The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases. BioMetals, 28(6), 975-986. doi:10.1007/s10534-015-9882-4.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-78F3-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-166D-B
Genre: Journal Article

Files

show Files
hide Files
:
2247118.pdf (Publisher version), 2MB
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Kuban-Jankowska, A., Author
Gorska, M., Author
Jaremko, L.1, Author              
Jaremko, M.1, Author              
Tuszynski, J. A., Author
Wozniak, M., Author
Affiliations:
1Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society, escidoc:578567              

Content

show
hide
Free keywords: Ferrous iron; Hydrogen peroxide; CD45; LAR; PTP1B
 Abstract: Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.

Details

show
hide
Language(s): eng - English
 Dates: 2015-09-252015-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10534-015-9882-4
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BioMetals
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 28 (6) Sequence Number: - Start / End Page: 975 - 986 Identifier: -