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Free keywords:
serial femtosecond crystallography; high-intensity phasing; radiation damage; electronic damage; X-ray free-electron lasers; high XFEL doses
Abstract:
Analysis of serial femtosecond crystallography data collected at the Linac Coherent Light Source using two distinct photon fluxes shows different degrees of ionization of Gd atoms bound to a lysozyme protein, due to electronic damage processes. The charge contrast on the heavy atoms is quantified using difference Fourier maps, and the way in which this could be applied to phasing is discussed.
