English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering.

Jain, R., Khan, N., Menzel, A., Rajkovic, I., Konrad, M., & Techert, S. (2016). Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering. European Biophysics Journal, 45(1), 81-89. doi:10.1007/s00249-015-1079-9.

Item is

Files

show Files
hide Files
:
2218438.pdf (Publisher version), 2MB
Name:
2218438.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2218438_Suppl.docx (Supplementary material), 2MB
Name:
2218438_Suppl.docx
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Jain, R.1, Author           
Khan, N.2, Author           
Menzel, A., Author
Rajkovic, I.1, Author           
Konrad, M.2, Author           
Techert, S.1, Author           
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society, ou_578564              
2Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

Content

show
hide
Free keywords: Protein conformations; Enzyme; Guanylate kinase (GMPK); Nucleotide kinase; Small-angle X-ray scattering (SAXS)
 Abstract: Bio-catalysis is the outcome of a subtle interplay between internal motions in enzymes and chemical kinetics. Small-angle X-ray scattering (SAXS) investigation of an enzyme's internal motions during catalysis offers an integral view of the protein's structural plasticity, dynamics, and function, which is useful for understanding allosteric effects and developing novel medicines. Guanylate kinase (GMPK) is an essential enzyme involved in the guanine nucleotide metabolism of unicellular and multicellular organisms. It is also required for the intracellular activation of numerous antiviral and anticancer purine nucleoside analog prodrugs. Catalytically active recombinant human GMPK (hGMPK) was purified for the first time and changes in the size and shape of open/closed hGMPK were tracked by SAXS. The binding of substrates (GMP + AMPPNP or Ap5G or GMP + ADP) resulted in the compaction of size and shape of hGMPK. The structural changes between open and completely closed hGMPK conformation were confirmed by observing differences in the hGMPK secondary structures with circular dichroism spectroscopy.

Details

show
hide
Language(s): eng - English
 Dates: 2015-10-072016-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/s00249-015-1079-9
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Biophysics Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 45 (1) Sequence Number: - Start / End Page: 81 - 89 Identifier: -