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  Cell substratum adhesion during early development of Dictyostelium discoideum

Tarantola, M., Bae, A., Fuller, D., Bodenschatz, E., Rappel, W.-J., & Loomis, W. F. (2014). Cell substratum adhesion during early development of Dictyostelium discoideum. PLoS ONE, 9, e106574-1-e106574-7. doi:10.1371/journal.pone.0106574.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-0EFF-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-0F00-1
Genre: Journal Article

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 Creators:
Tarantola, Marco1, Author              
Bae, Albert1, Author              
Fuller, Danny, Author
Bodenschatz, Eberhard1, Author              
Rappel, Wouter-Jan, Author
Loomis, William F., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, escidoc:2063287              

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 Abstract: Vegetative and developed amoebae of Dictyostelium discoideum gain traction and move rapidly on a wide range of substrata without forming focal adhesions. We used two independent assays to quantify cell-substrate adhesion in mutants and in wild-type cells as a function of development. Using a microfluidic device that generates a range of hydrodynamic shear stress, we found that substratum adhesion decreases at least 10 fold during the first 6 hr of development of wild type cells. This result was confirmed using a single-cell assay in which cells were attached to the cantilever of an atomic force probe and allowed to adhere to untreated glass surfaces before being retracted. Both of these assays showed that the decrease in substratum adhesion was dependent on the cAMP receptor CAR1 which triggers development. Vegetative cells missing talin as the result of a mutation in talA exhibited slightly reduced adhesive properties compared to vegetative wildtype cells. In sharp contrast to wild-type cells, however, these talA mutant cells did not show further reduction of adhesion during development such that after 5 hr of development they were significantly more adhesive than developed wild type cells. In addition, both assays showed that substrate adhesion was reduced in 0 hr cells when the actin cytoskeleton was disrupted by latrunculin. Consistent with previous observations, substrate adhesion was also reduced in 0 hr cells lacking the membrane proteins SadA or SibA as the result of mutations in sadA or sibA. However, there was no difference in the adhesion properties between wild type AX3 cells and these mutant cells after 6 hr of development, suggesting that neither SibA nor SadA play an essential role in substratum adhesion during aggregation. Our results provide a quantitative framework for further studies of cell substratum adhesion in Dictyostelium.

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Language(s): eng - English
 Dates: 2014-09-23
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 702118
DOI: 10.1371/journal.pone.0106574
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Title: PLoS ONE
  Alternative Title : PLoS ONE
Source Genre: Journal
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Pages: - Volume / Issue: 9 Sequence Number: - Start / End Page: e106574-1 - e106574-7 Identifier: -