English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics.

Schneider, J., Zahn, J., Maglione, M., Sigrist, S. J., Marquard, J., Chojnacki, J., et al. (2015). Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics. Nature Methods, 12(9), 827-830. doi:10.1038/nmeth.3481.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-D460-F Version Permalink: https://hdl.handle.net/21.11116/0000-0002-A715-A
Genre: Journal Article

Files

show Files
hide Files
:
2173123.pdf (Publisher version), 755KB
 
File Permalink:
-
Name:
2173123.pdf
Description:
-
OA-Status:
Visibility:
Restricted (UNKNOWN id 303; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_1.html (Supplementary material), 74KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DE8-C
Name:
2173123_Suppl_1.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_2.html (Supplementary material), 72KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DE9-A
Name:
2173123_Suppl_2.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_3.html (Supplementary material), 73KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DEA-8
Name:
2173123_Suppl_3.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_4.html (Supplementary material), 78KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DEB-6
Name:
2173123_Suppl_4.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123-Suppl_5.html (Supplementary material), 78KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DEC-4
Name:
2173123-Suppl_5.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_6.html (Supplementary material), 78KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DED-2
Name:
2173123_Suppl_6.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_7.html (Supplementary material), 78KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DEE-F
Name:
2173123_Suppl_7.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_8.html (Supplementary material), 78KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DEF-D
Name:
2173123_Suppl_8.html
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
2173123_Suppl_9.pdf (Supplementary material), 787KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0028-5DF0-7
Name:
2173123_Suppl_9.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://www.nature.com/nmeth/journal/v12/n9/pdf/nmeth.3481.pdf (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Schneider, J., Author
Zahn, J., Author
Maglione, M., Author
Sigrist, S. J., Author
Marquard, J., Author
Chojnacki, J., Author
Kräusslich, H. G., Author
Sahl, S. J.1, Author           
Engelhardt, J.1, Author           
Hell, S. W.1, Author           
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: Super-resolution microscopy, Confocal microscopy, Fluorescence imaging
 Abstract: Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution.

Details

show
hide
Language(s): eng - English
 Dates: 2015-07-272015-09
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nmeth.3481
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 12 (9) Sequence Number: - Start / End Page: 827 - 830 Identifier: -