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Abstract:
Visualizing the microbial cell surface by microscopy has been and still is a
challenge. Fluorescence light-optical methods locate structures with
accuracy in the lower nanometer range, scanning force microscopes
record corrugations with angstrom sensitivity, and electron microscopy
provides near atomic resolution of protein complexes. But if the surface
of intact cells is to be investigated we are limited by several restrictions.
What can microscopy achieve and which method is promising?
