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  N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.

Nishibuchi, G., Machida, S., Osakabe, A., Murakoshi, H., Hiragami-Hamada, K., Nakagawa, R., et al. (2014). N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity. Nucleic Acids Research, 42(20), 12498-12511. doi:10.1093/nar/gku995.

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Nishibuchi, G., Autor
Machida, S., Autor
Osakabe, A., Autor
Murakoshi, H., Autor
Hiragami-Hamada, K.1, Autor           
Nakagawa, R., Autor
Fischle, W.1, Autor           
Nishimura, Y., Autor
Kurumizaka, H., Autor
Tagami, H., Autor
Nakayama, J., Autor
Affiliations:
1Research Group of Chromatin Biochemistry, MPI for Biophysical Chemistry, Max Planck Society, ou_578604              

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 Zusammenfassung: Heterochromatin protein 1 (HP1) is an evolutionarily conserved chromosomal protein that binds to lysine 9-methylated histone H3 (H3K9me), a hallmark of heterochromatin. Although HP1 phosphorylation has been described in several organisms, the biological implications of this modification remain largely elusive. Here we show that HP1's phosphorylation has a critical effect on its nucleosome binding properties. By in vitro phosphorylation assays and conventional chromatography, we demonstrated that casein kinase II (CK2) is the kinase primarily responsible for phosphorylating the N-terminus of human HP1α. Pull-down assays using in vitro-reconstituted nucleosomes showed that unmodified HP1α bound H3K9-methylated and H3K9-unmethylated nucleosomes with comparable affinity, whereas CK2-phosphorylated HP1α showed a high specificity for H3K9me3-modified nucleosomes. Electrophoretic mobility shift assays showed that CK2-mediated phosphorylation diminished HP1α's intrinsic DNA binding, which contributed to its H3K9me-independent nucleosome binding. CK2-mediated phosphorylation had a similar effect on the nucleosome-binding specificity of fly HP1a and S. pombe Swi6. These results suggested that HP1 phosphorylation has an evolutionarily conserved role in HP1's recognition of H3K9me-marked nucleosomes.

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Sprache(n): eng - English
 Datum: 2014-10-202014-11-10
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1093/nar/gku995
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Titel: Nucleic Acids Research
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 42 (20) Artikelnummer: - Start- / Endseite: 12498 - 12511 Identifikator: -