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  The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3′ end formation

Holbein, S., Scola, S., Loll, B., Dichtl, B. S., Hübner, W., Meinhart, A., et al. (2011). The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3′ end formation. PLoS One, 6(12): e29139, pp. 1-10. doi:10.1371/journal.pone.0029139.

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Genre: Journal Article
Alternative Title : The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3′ end formation

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Holbein, Sandra, Author
Scola, Simonetta, Author
Loll, Bernhard1, Author           
Dichtl, Beatriz Solange, Author
Hübner, Wolfgang, Author
Meinhart, Anton1, Author           
Dichtl, Bernhard, Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Cleavage factor IA (CF IA), cleavage and polyadenylation factor (CPF), constitute major protein complexes required for pre−mRNA 3' end formation in yeast. The Clp1 protein associates with Pcf11, Rna15 and Rna14 in CF IA but its functional role remained unclear. Clp1 carries an evolutionarily conserved P−loop motif that was previously shown to bind ATP. Interestingly, human and archaean Clp1 homologues, but not the yeast protein, carry 5' RNA kinase activity. We show that depletion of Clp1 in yeast promoted defective 3' end formation and RNA polymerase II termination; however, cells expressing Clp1 with mutant P−loops displayed only minor defects in gene expression. Similarly, purified and reconstituted mutant CF IA factors that interfered with ATP binding complemented CF IA depleted extracts in coupled in vitro transcription/3' end processing reactions. We found that Clp1 was required to assemble recombinant CF IA and that certain P−loop mutants failed to interact with the CF IA subunit Pcf11. In contrast, mutations in Clp1 enhanced binding to the 3' endonuclease Ysh1 that is a component of CPF. Our results support a structural role for the Clp1 P−loop motif. ATP binding by Clp1 likely contributes to CF IA formation and cross−factor interactions during the dynamic process of 3' end formation

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Language(s): eng - English
 Dates: 2011-07-092011-11-212011-12-22
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 664468
DOI: 10.1371/journal.pone.0029139
Other: 7733
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 6 (12) Sequence Number: e29139 Start / End Page: 1 - 10 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850