English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Time-resolved protein nanocrystallography using an X-ray free-electron laser

Aquila, A., Hunter, M. S., Doak, R. B., Kirian, R. A., Fromme, P., White, T. A., et al. (2012). Time-resolved protein nanocrystallography using an X-ray free-electron laser. Optics Express, 20(3), 2706-2716. doi:10.1364/OE.20.002706.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Time-resolved protein nanocrystallography using an X-ray free-electron laser

Files

show Files
hide Files
:
OptExpress_20_2012_2706.pdf (Any fulltext), 3MB
 
File Permalink:
-
Name:
OptExpress_20_2012_2706.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.1364/OE.20.002706 (Any fulltext)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Aquila, Andrew, Author
Hunter, Mark S., Author
Doak, R. Bruce1, Author           
Kirian, Richard A., Author
Fromme, Petra, Author
White, Thomas A., Author
Andreasson, Jakob, Author
Arnlund, David, Author
Bajt, Saša, Author
Barends, Thomas1, Author           
Barthelmess, Miriam, Author
Bogan, Michael J., Author
Bostedt, Christoph, Author
Bottin, Hervé, Author
Bozek, John D., Author
Caleman, Carl, Author
Coppola, Nicola, Author
Davidsson, Jan, Author
DePonte, Daniel P., Author
Elser, Veit, Author
Epp, Sascha W., AuthorErk, Benjamin1, Author           Fleckenstein, Holger, AuthorFoucar, Lutz1, Author           Frank, Matthias, AuthorFromme, Raimund, AuthorGraafsma, Heinz, AuthorGrotjohann, Ingo, AuthorGumprecht, Lars, AuthorHajdu, Janos, AuthorHampton, Christina Y., AuthorHartmann, Andreas, AuthorHartmann, Robert, AuthorHau−Riege, Stefan P., AuthorHauser, Günter, AuthorHirsemann, Helmut, AuthorHoll, Peter, AuthorHolton, James M., AuthorHömke, André, AuthorJohansson, Linda, AuthorKimmel, Nils, AuthorKassemeyer, Stephan1, Author           Krasniqi, Faton1, Author           Kühnel, Kai−Uwe, AuthorLiang, Mengning, AuthorLomb, Lukas1, Author           Malmerberg, Erik, AuthorMarchesini, Stefano, AuthorMartin, Andrew V., AuthorMaia, Filipe R.N.C., AuthorMesserschmidt, Marc, AuthorNass, Karol1, Author           Reich, Christian, AuthorNeutze, Richard, AuthorRolles, Daniel1, Author           Rudek, Benedikt1, Author           Rudenko, Artem, AuthorSchlichting, Ilme1, Author           Schmidt, Carlo, AuthorSchmidt, Kevin E., AuthorSchulz, Joachim, AuthorSeibert, M. Marvin, AuthorShoeman, Robert L.1, Author           Sierra, Raymond G., AuthorSoltau, Heike, AuthorStarodub, Dmitri, AuthorStellato, Francesco, AuthorStern, Stephan, AuthorStrüder, Lothar, AuthorTimneanu, Nicusor, AuthorUllrich, Joachim, AuthorWang, Xiaoyu, AuthorWilliams, Garth J., AuthorWeidenspointner, Georg, AuthorWeierstall, Uwe, AuthorWunderer, Cornelia B., AuthorBarty, Anton, AuthorSpence, John C. H., AuthorChapman, Henry N., Author more..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: -
 Abstract: We demonstrate the use of an X−ray free electron laser synchronized with an optical pump laser to obtain X−ray diffraction snapshots from the photoactivated states of large membrane protein complexes in the form of nanocrystals flowing in a liquid jet. Light−induced changes of Photosystem I−Ferredoxin co−crystals were observed at time delays of 5 to 10 μs after excitation. The result correlates with the microsecond kinetics of electron transfer from Photosystem I to ferredoxin. The undocking process that follows the electron transfer leads to large rearrangements in the crystals that will terminally lead to the disintegration of the crystals. We describe the experimental setup and obtain the first timeresolved femtosecond serial X−ray crystallography results from an irreversible photo−chemical reaction at the Linac Coherent Light Source. This technique opens the door to time−resolved structural studies of reaction dynamics in biological systems

Details

show
hide
Language(s): eng - English
 Dates: 2011-11-112011-12-182012-01-30
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Optics Express
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 20 (3) Sequence Number: - Start / End Page: 2706 - 2716 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918