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  Resolution enhancement of suspended microchannel resonators for weighing of biomolecular complexes in solution.

Modena, M. M., Wang, Y., Riedel, D., & Burg, T. P. (2014). Resolution enhancement of suspended microchannel resonators for weighing of biomolecular complexes in solution. Lab on a Chip, 14(2), 342-350. doi:10.1039/C3LC51058A.

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 Urheber:
Modena, M. M.1, Autor           
Wang, Y.1, Autor           
Riedel, D.2, Autor           
Burg, T. P.1, Autor           
Affiliations:
1Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society, ou_578602              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Zusammenfassung: We introduce the use of correlation analysis to extend the dynamic range of Suspended Micro- and Nanochannel Resonator (SMR/SNR) mass sensors by over five orders of magnitude. This method can analyze populations of particles flowing through an embedded channel micromechanical resonator, even when the individual particle masses are far below the noise floor. To characterize the method, we measured the mass of polystyrene nanoparticles with 300 zg resolution. As an application, we monitored the time course of insulin amyloid formation from pre-fibrillar aggregates to mature fibrils of 15 MDa average mass. Results were compared with Thioflavin-T (ThT) assays and electron microscopy (EM). Mass measurements offer additional information over ThT during the fluorescent inaccessible lag period, and the average fibril dimensions calculated from the mass signal are in good accordance with EM. In the future, we envision that more detailed modeling will allow the computational deconvolution of multicomponent samples, enabling the mass spectrometric characterization of a variety of biomolecular complexes, small organelles, and nanoparticles in solution.

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Sprache(n): eng - English
 Datum: 2013-11-182014
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1039/C3LC51058A
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Titel: Lab on a Chip
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 14 (2) Artikelnummer: - Start- / Endseite: 342 - 350 Identifikator: -