English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Increased proteome coverage by combining PAGE and peptide isoelectric focusing: Comparative study of gel-based separation approaches.

Atanassov., I., & Urlaub, H. (2013). Increased proteome coverage by combining PAGE and peptide isoelectric focusing: Comparative study of gel-based separation approaches. Proteomics, 13(20), 2947-2955. doi:10.1002/pmic.201300035.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-7925-E Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-CB69-4
Genre: Journal Article

Files

show Files
hide Files
:
1850307.pdf (Publisher version), 543KB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0014-7928-8
Name:
1850307.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-
:
1850307_Supplement_1.ppt (Supplementary material), 13MB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0014-7929-6
Name:
1850307_Supplement_1.ppt
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/vnd.ms-powerpoint / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201300035/pdf (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Atanassov., I.1, Author           
Urlaub, H.1, Author           
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: -
 Abstract: The in-depth analysis of complex proteome samples requires fractionation of the sample into subsamples prior to LC-MS/MS in shotgun proteomics experiments. We have established a 3D workflow for shotgun proteomics that relies on protein separation by 1D PAGE, gel fractionation, trypsin digestion, and peptide separation by in-gel IEF, prior to RP-HPLC-MS/MS. Our results show that applying peptide IEF can significantly increase the number of proteins identified from PAGE subfractionation. This method delivers deeper proteome coverage and provides a large degree of flexibility in experimentally approaching highly complex mixtures by still relying on protein separation according to molecular weight in the first dimension.

Details

show
hide
Language(s): eng - English
 Dates: 2013-10-082013-10
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/pmic.201300035
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proteomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 (20) Sequence Number: - Start / End Page: 2947 - 2955 Identifier: -