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  Abnormal Physiological and Molecular Mutant Phenotypes Link Chloroplast Polynucleotide Phosphorylase to the Phosphorus Deprivation Response in Arabidopsis

Marchive, C., Yehudai-Resheff, S., Germain, A., Fei, Z. J., Jiang, X. S., Judkins, J., et al. (2009). Abnormal Physiological and Molecular Mutant Phenotypes Link Chloroplast Polynucleotide Phosphorylase to the Phosphorus Deprivation Response in Arabidopsis. Plant Physiology, 151(2), 905-924. doi:10.1104/pp.109.145144.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2550-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-84C2-B
Genre: Journal Article

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Marchive-2009-Abnormal Physiologic.pdf (Any fulltext), 4MB
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 Creators:
Marchive, C.1, Author
Yehudai-Resheff, S.1, Author
Germain, A.1, Author
Fei, Z. J.1, Author
Jiang, X. S.1, Author
Judkins, J.1, Author
Wu, H.1, Author
Fernie, A. R.2, Author              
Fait, A.2, Author              
Stern, D. B.1, Author
Affiliations:
1External Organizations, escidoc:persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, escidoc:1753339              

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Free keywords: programmed cell-death methylerythritol phosphate-pathway rna-binding proteins transcription factor er-body starvation responses poly(a) polymerase root architecture acid biosynthesis escherichia-coli
 Abstract: A prominent enzyme in organellar RNA metabolism is the exoribonuclease polynucleotide phosphorylase (PNPase), whose reversible activity is governed by the nucleotide diphosphate-inorganic phosphate ratio. In Chlamydomonas reinhardtii, PNPase regulates chloroplast transcript accumulation in response to phosphorus (P) starvation, and PNPase expression is repressed by the response regulator PSR1 (for PHOSPHORUS STARVATION RESPONSE1) under these conditions. Here, we investigated the role of PNPase in the Arabidopsis (Arabidopsis thaliana) P deprivation response by comparing wild-type and pnp mutant plants with respect to their morphology, metabolite profiles, and transcriptomes. We found that P-deprived pnp mutants develop aborted clusters of lateral roots, which are characterized by decreased auxin responsiveness and cell division, and exhibit cell death at the root tips. Electron microscopy revealed that the collapse of root organelles is enhanced in the pnp mutant under P deprivation and occurred with low frequency under P-replete conditions. Global analyses of metabolites and transcripts were carried out to understand the molecular bases of these altered P deprivation responses. We found that the pnp mutant expresses some elements of the deprivation response even when grown on a full nutrient medium, including altered transcript accumulation, although its total and inorganic P contents are not reduced. The pnp mutation also confers P status-independent responses, including but not limited to stress responses. Taken together, our data support the hypothesis that the activity of the chloroplast PNPase is involved in plant acclimation to P availability and that it may help maintain an appropriate balance of P metabolites even under normal growth conditions.

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Language(s): eng - English
 Dates: 2009-08-282009
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000270389500034
DOI: 10.1104/pp.109.145144
ISSN: 0032-0889 (Print)0032-0889 (Linking)
URI: ://000270389500034http://www.jstor.org/stable/pdfplus/40537821.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 151 (2) Sequence Number: - Start / End Page: 905 - 924 Identifier: ISSN: 0032-0889
CoNE: http://pubman.mpdl.mpg.de/cone/journals/resource/991042744294438