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  Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches

Gueorguieva, L., Vallejo, L. F., Rinas, U., & Seidel-Morgenstern, A. (2006). Discontinuous and continuous separation of the monomeric and dimeric forms of human bone morphogenetic protein-2 from renaturation batches. Journal of Chromatography A, 1135(2), 142-150. doi:10.1016/j.chroma.2006.08.061.

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Gueorguieva, L.1, 2, Autor           
Vallejo, L. F.3, Autor
Rinas, U.3, Autor
Seidel-Morgenstern, A.1, 2, Autor           
Affiliations:
1Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
2Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
3Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany, ou_persistent22              

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Schlagwörter: Bone morphogenetic protein-2; Gradient elution; Continuous counter-current separation; Step gradients
 Zusammenfassung: Bone morphogenetic protein-2 (BMP-2) is one of the most interesting of the approximately 14 BMPs which belong to the transforming-growth-factor-β (TGF-β) superfamily. BMP-2 induces bone formation and thus plays an important role as a pharmaceutical protein. Recently, rhBMP-2 has been produced in form of inactive inclusion bodies in Escherichia coli. After solubilization and renaturation the biologically active dimeric form of rhBMP-2 can be generated. However, inactive monomers of BMP-2 are also formed during the renaturation process which must be separated from the active dimeric BMP-2. The purpose of this paper is to present: (a) results of an experimental study of a chromatographic separation of the monomeric and dimeric forms; and (b) a concept for a continuous counter-current simulated moving bed (SMB) process. The capacity of heparin as stationary phase was estimated for different salt concentrations in the mobile phase. A simulation study of a three-zone SMB process was performed applying a two step salt gradient. The results reveal the potential of the process for the purification of the dimeric BMP-2. © 2006 Published by Elsevier B.V. [accessed 2013 November 27th]

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Sprache(n): eng - English
 Datum: 2006
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 290788
DOI: 10.1016/j.chroma.2006.08.061
Anderer: 38/06
 Art des Abschluß: -

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Titel: Journal of Chromatography A
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : Elsevier
Seiten: - Band / Heft: 1135 (2) Artikelnummer: - Start- / Endseite: 142 - 150 Identifikator: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1