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  Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography

Opitz, L., Hohlweg, J., Reichl, U., & Wolff, M. W. (2009). Purification of cell culture-derived influenza virus A/Puerto Rico/8/34 by membrane-based immobilized metal affinity chromatography. Journal of Virological Methods, 161(2), 312-316. doi:10.1016/j.jviromet.2009.06.025.

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 Creators:
Opitz, L.1, Author           
Hohlweg, J.1, 2, Author           
Reichl, U.1, 3, Author           
Wolff, M. W.1, 3, Author           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Fachhochschule Südwestfalen, Iserlohn, persistent:22              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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 Abstract: The presented study focuses on the feasibility of immobilized metal affinity chromatography for purification of Madin Darby canine kidney cell culture-derived influenza virus particles. Therefore, influenza virus A/Puerto Rico/8/34 was screened for adsorption to different transition metal ions attached to iminodiacetic acid. Subsequently, capturing of the same virus strain using zinc-modified iminodiacetic acid membrane adsorbers was characterized regarding viral recoveries, host cell nucleic acid and total protein depletion as well as zinc ion leaching. In addition, the effect of the imidazole proton pump on virus stability was studied based on the hemagglutination activity. During adsorption in the presence of 1 M sodium chloride the majority of virus particles were recovered in the product (64 % Hemagglutination activity). Host cell nucleic acid and total protein content were reduced to approximately 7 % and 26%, respectively. This inexpensive and rapid method was applied reproducibly for influenza virus A/Puerto Rico/8/34 preparations on the laboratory scale. However, preliminary results with other virus strains indicated clearly a strong strain dependency for viral adsorption. Copyright © 2009 Elsevier B.V. All rights reserved. [Accessed September 16, 2009]

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Language(s): eng - English
 Dates: 2009
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 436040
Other: 17/09 b
DOI: 10.1016/j.jviromet.2009.06.025
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Title: Journal of Virological Methods
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 161 (2) Sequence Number: - Start / End Page: 312 - 316 Identifier: ISSN: 0166-0934
CoNE: https://pure.mpg.de/cone/journals/resource/954925482627