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  How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications

Andexer, J. N., von Langermann, J., Kragl, U., & Pohl, M. (2009). How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications. Trends in Biotechnology, 27(10), 599-607. doi:10.1016/j.tibtech.2009.07.005.

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Andexer, J. N.1, 2, Autor
von Langermann, J.3, 4, Autor           
Kragl, U.4, Autor
Pohl, M.1, Autor
Affiliations:
1Universität Düsseldorf, Institut für Molekulare Enzymtechnologie, Jülich, Germany, ou_persistent22              
2University of Cambridge, Department of ChemistryLensfield Road, Cambridge, CB2 1EW, United Kingdom, ou_persistent22              
3Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
4Universität Rostock, Institut für Chemie, Rostock, Germany, ou_persistent22              

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 Zusammenfassung: During the last decades, enzymes became very versatile catalysts for a variety of reactions including natural and unnatural compounds. However, many enzyme-catalysed reactions suffer from diverse restrictions because of limitations related to process parameters or the enzyme. The understanding and overcoming of those undesired side effects is therefore mandatory for the implementation of optimal process parameters. To achieve this aim, various methods from molecular biology and reaction engineering can be employed. By focusing on the hydroxynitrile lyase-catalysed synthesis of enantiopure cyanohydrins, we give an overview of strategies to improve commercially utilized enzymes and to suppress non-enzymatic reactions. Particular emphasis is placed on the necessity to combine approaches from different fields, such as enzyme engineering and reaction engineering. Copyright © 2009 Elsevier Ltd All rights reserved. [accessed September 17, 2009]

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Sprache(n): eng - English
 Datum: 2009
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 435956
DOI: 10.1016/j.tibtech.2009.07.005
Anderer: 24/09
DOI: 10.1016/j.tibtech.2009.07.005
 Art des Abschluß: -

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Titel: Trends in Biotechnology
  Andere : Trends Biotechnol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam, Netherlands : Elsevier Current Trends
Seiten: - Band / Heft: 27 (10) Artikelnummer: - Start- / Endseite: 599 - 607 Identifikator: ISSN: 0167-7799
CoNE: https://pure.mpg.de/cone/journals/resource/110984180788411