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  Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography - Part II: Process design and experimental validation

Gueorguieva, L., Palani, S., Rinas, U., Jayaraman, G., & Seidel-Morgenstern, A. (2011). Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography - Part II: Process design and experimental validation. Journal of Chromatography A, 1218(37), 6402-6411. doi:10.1016/j.chroma.2011.07.008.

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Gueorguieva, L.1, Autor           
Palani, S.2, 3, Autor           
Rinas, U.4, 5, Autor
Jayaraman, G.3, Autor
Seidel-Morgenstern, A.1, 2, Autor           
Affiliations:
1Otto-von-Guericke-Universität Magdeburg, External Organizations, ou_1738156              
2Physical and Chemical Foundations of Process Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738150              
3Indian Institute of Technology-Madras, Dep. of Biotechnology, Chennai, India, persistent:22              
4Leibniz University of Hanover, Institute of Technical Chemistry–Life Science, Hannover, Germany, ou_persistent22              
5Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany, ou_persistent22              

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Schlagwörter: streptokinase; hydrophobic interaction chromatography; two-step gradient open-loop simulated moving bed chromatography
 Zusammenfassung: In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two–step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified E. coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated. Copyright © 2011 Published by Elsevier B.V. [accessed September 1st 2011]

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Sprache(n): eng - English
 Datum: 2011
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 570418
DOI: 10.1016/j.chroma.2011.07.008
 Art des Abschluß: -

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Titel: Journal of Chromatography A
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Amsterdam : Elsevier
Seiten: - Band / Heft: 1218 (37) Artikelnummer: - Start- / Endseite: 6402 - 6411 Identifikator: ISSN: 0021-9673
CoNE: https://pure.mpg.de/cone/journals/resource/954925527837_1