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Free keywords:
streptokinase; hydrophobic interaction chromatography; two-step gradient open-loop simulated moving bed chromatography
Abstract:
In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two–step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified E. coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated.
Copyright © 2011 Published by Elsevier B.V. [accessed September 1st 2011]
