Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Cell culture-based influenza virus production: suspension or adherent producer cell lines?

Genzel, Y., Lohr, V., Behrendt, I., Scharfenberg, K., Kamen, A., Schiedner, G., et al. (2012). Cell culture-based influenza virus production: suspension or adherent producer cell lines?. Talk presented at Cell line development and engineering 2012. Cologne, Germany. 2012-02-13 - 2012-02-17.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Genzel, Yvonne1, Autor           
Lohr, Verena1, Autor           
Behrendt, Ilona1, Autor           
Scharfenberg, Klaus, Autor
Kamen, Amine, Autor
Schiedner, Gudrun, Autor
Jordan, Ingo, Autor
Reichl, Udo1, 2, Autor           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2Otto-von-Guericke-Universität Magdeburg, ou_1738156              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Various cell lines are currently being evaluated for influenza virus production and process performance to find alternatives to the egg-based vaccine manufacturing. Choosing between suspension and adherent cell lines is not easy and requires evaluation of different process concepts. Here, we present a comparison of adherent cell lines (MDCK, Vero) and suspension cell lines (MDCK.SUS2, AGE1.CR, HEK293, CAP) to give insights into the complexity of this choice. The cells were cultivated in various cultivation systems including roller bottles, shaker flasks, small scale stirred tank reactors, and wave bioreactors. Different cultivation strategies were investigated (batch (with or without medium exchange), perfusion), cultivating each cell line in its appropriate medium. Infection with several influenza virus strains using different infection conditions (MOI (multiplicity of infection), trypsin activity) helped to improve virus yields. Data on metabolism, cell growth and virus titer will be discussed for several bioreactor cultivations. It can be seen that a simple screening of cell lines would not lead to satisfactory results concerning the choice of adherent versus suspension cell-based processes and optimum cultivation conditions. In particular, each of the cell lines investigated can produce most influenza strains in acceptable titers after careful adaptation and selection of appropriate process conditions. It remains however unclear, why some virus strains cannot be produced by specific cell lines. Because of this it seems advisable, to have several alternatives for influenza vaccine manufacturing in case of a pandemic threat.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2012
 Publikationsstatus: Keine Angabe
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 610106
 Art des Abschluß: -

Veranstaltung

einblenden:
ausblenden:
Titel: Cell line development and engineering 2012
Veranstaltungsort: Cologne, Germany
Start-/Enddatum: 2012-02-13 - 2012-02-17

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle

einblenden: