NMR in the SPINE structural proteomics project.

Ab, E.; Atkinson, A. R.; Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Brunner, K.; Diercks, T.; Dötsch, V.; Engelke, F.; Folkers, G. E.; Griesinger, C.; Gronwald, W.; Günther, U.; Habeck, M.; De Jong, R. N.; Kalbitzer, H. R.; Kieffer, B.; Leeflang, B. R.; Loss, S.; Luchinat, C.; Marquardsen, T.; Moskau, D.; Neidig, K. P.; Nilges, M.; Piccioli, M.; Pierattelli, R.; Rieping, W.; Schippmann, T.; Schwalbe, H.; Travé, G.; Trenner, J.; Wöhnert, J.; Zweckstetter, M.; Kaptein, R.

doi:10.1107/S0907444906032070

Local TagsRelease HistoryDetailsSummary

NMR in the SPINE structural proteomics project.

Ab, E., Atkinson, A. R., Banci, L., Bertini, I., Ciofi-Baffoni, S., Brunner, K., et al. (2006). NMR in the SPINE structural proteomics project. Acta Crystallographica Section D-Biological Crystallography, 62(10), 1150-1161. doi:10.1107/S0907444906032070.

Item is Released

show all hide all

Basic

show hide

Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000E-7096-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-C7DB-4

Genre: Journal Article

Files

show Files

hide Files

:

1587325.pdf (Publisher version), 2MB

File Permalink:
-

Name:
1587325.pdf

Description:
-

OA-Status:

Visibility:
Restricted (UNKNOWN id 303; )

MIME-Type / Checksum:
application/pdf

Technical Metadata:

Copyright Date:
-

Copyright Info:
-

License:
-

Locators

show

hide

Locator:
http://onlinelibrary.wiley.com/doi/10.1107/S0907444906032070/pdf (Publisher version) Open Access status unknown

Description:
-

OA-Status:

Creators

show

hide

Creators:
Ab, E., Author
Atkinson, A. R., Author
Banci, L., Author
Bertini, I., Author
Ciofi-Baffoni, S., Author
Brunner, K., Author
Diercks, T., Author
Dötsch, V., Author
Engelke, F., Author
Folkers, G. E., Author
Griesinger, C.¹, Author
Gronwald, W., Author
Günther, U., Author
Habeck, M., Author
De Jong, R. N., Author
Kalbitzer, H. R., Author
Kieffer, B., Author
Leeflang, B. R., Author
Loss, S., Author
Luchinat, C., Author
Marquardsen, T., AuthorMoskau, D., AuthorNeidig, K. P., AuthorNilges, M., AuthorPiccioli, M., AuthorPierattelli, R., AuthorRieping, W., AuthorSchippmann, T., AuthorSchwalbe, H., AuthorTravé, G., AuthorTrenner, J., AuthorWöhnert, J., AuthorZweckstetter, M.², Author Kaptein, R., Author more..

Affiliations:
1Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567
2Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571

Content

show

hide

Free keywords: -

Abstract: This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe (SPINE) consortium. Focusing on the development of high-throughput (HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless13C-direct detection methods and inferential structure determinations (ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.

Details

show

hide

Language(s): eng - English

Dates: Published Online: 2006-10-04Date issued: 2006-10

Publication Status: Issued

Pages: -

Publishing info: -

Table of Contents: -

Rev. Type: Peer

Identifiers: DOI: 10.1107/S0907444906032070

Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show

hide

Title: Acta Crystallographica Section D-Biological Crystallography

Source Genre: Journal

Creator(s):

Affiliations:

Publ. Info: -

Pages: - Volume / Issue: 62 (10) Sequence Number: - Start / End Page: 1150 - 1161 Identifier: -