Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  Assembly and characterization of protein multi-layer systems

Christensen, D. (2004). Assembly and characterization of protein multi-layer systems. Master Thesis, Johannes Gutenberg-Universität, Mainz.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-6080-2 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-6081-F
Genre: Hochschulschrift

Dateien

einblenden: Dateien
ausblenden: Dateien
:
CHRIST~1.PDF (beliebiger Volltext), 3MB
 
Datei-Permalink:
-
Name:
CHRIST~1.PDF
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Polymer Research, MMPL; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
eDoc_access: INSTITUT
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Christensen, Danica1, Autor           
Affiliations:
1MPI for Polymer Research, Max Planck Society, ou_1309545              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Abstract: Biocompatible multi-layer architectures are of great interest for the construction of biosensor applications. We have established a system that is a multi-layer protein architecture with alternating layers of streptavidin (SA) and biotinylated immunoglobulin G (B-IgG). The layer building properties of this protein multi-layer architecture has been studied in situ using the surface plasmon resonance spectroscopy (SPR). Studies were also done using neutron reflectivity to investigate the surface architecture of the multi-layers. The multi-layer architecture has been shown to assemble with a linear growth of the multi-layer thickness with respect to the number of layers deposited. Experiments conducted using SPR have also shown that the multi-layers are stable against free biotin attack and the assembly of the multi-layers is very reproducible. The effects of using SA versus avidin to build the multi-layer system were studied using SPR. Two immobilization techniques for the attachment of the first SA layer to the metal surface were compared with SPR; the first using covalent bonding between the SA and a thiol derivative self-assembled to the surface, the second technique used the binding affinity of SA and biotin to bind the SA directly to a biotinylated thiol on the gold surface. The fluorescence intensity of Alexa fluoro labeled streptavidin (AFSA) was investigated using Surface Plasmon Fluorescence Spectroscopy (SPFS). The effect of adding additional non-labeled protein layers to a layer of AFSA that was placed close to the gold surface, about 3 nm, and a second layer a bit further away from the metal surface, at 15 nm, on the fluorescence intensity was also investigated using SPFS.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2004
 Publikationsstatus: Angenommen
 Seiten: -
 Ort, Verlag, Ausgabe: Mainz : Johannes Gutenberg-Universität
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 205896
Anderer: D 6/04
 Art des Abschluß: Master

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle

einblenden: